Development and comparison of duplex crystal digital PCR and qPCR assays for the detection of Muscovy duck parvovirus and goose parvovirus.

Huixin Liu, Kaichuang Shi, Yanwen Yin, Shuping Feng, Feng Long, Hongbin Si, Bin Xiang, Liangyu Yang

Abstract readComparative Study

In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Huixin LiuCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, Guangxi, China; College of Animal Science and Technology, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi grass station, Guangxi University, Nanning 530004, Guangxi, China.

Kaichuang ShiGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, Guangxi, China; College of Animal Science and Technology, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi grass station, Guangxi University, Nanning 530004, Guangxi, China. Electronic address: shikaichuang@126.com.

Yanwen YinGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, Guangxi, China.

Shuping FengGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, Guangxi, China.

Feng LongGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, Guangxi, China.

Hongbin SiGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, Guangxi, China; College of Animal Science and Technology, Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, Guangxi grass station, Guangxi University, Nanning 530004, Guangxi, China.

Bin XiangCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Center for Poultry Disease Control and Prevention, Yunnan Agricultural University, Kunming, Yunnan 650201, China.

Liangyu YangCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan 650201, China; Center for Poultry Disease Control and Prevention, Yunnan Agricultural University, Kunming, Yunnan 650201, China. Electronic address: 1993009@ynau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Muscovy duck parvovirus (MDPV) and Goose parvovirus (GPV) are highly contagious waterfowl viruses causing significant harm to the poultry industry. This study designed primers and probes targeting the non-structural genomic region of MDPV and GPV, optimizing amplification for both quantitative polymerase chain reaction (qPCR) and duplex crystal digital polymerase chain reaction (dPCR). The resulting duplex crystal dPCR method showed strong specificity, detecting MDPV and GPV without cross-reactivity with other waterfowl viruses. Sensitivity tests revealed that the lower detection limit for qPCR was 11.5 copies/μL, while duplex crystal dPCR achieved a significantly lower detection limit of 0.3 copies/μL. This indicates that dPCR is approximately 38.3-fold more sensitive than qPCR in detecting MDPV and GPV The method demonstrated good repeatability with variation coefficients below 8.0%. In 52 clinical samples from Guangxi, the positive rates for MDPV and GPV were 15.38% and 23.08%, respectively, with one co-infection. This study confirms that duplex crystal dPCR is a specific, sensitive, and efficient technique for detecting MDPV and GPV, especially in the detection of samples containing low concentrations of viruses.

Indexed as

DucksParvoviridae InfectionsParvovirinaeParvovirusPolymerase Chain ReactionPoultry DiseasesReal-Time Polymerase Chain ReactionAnimalsSensitivity and SpecificityDetection of samples containing low concentrations of virusesDuplex crystal dPCRGoose parvovirusMuscovy duck parvovirusQuantitative polymerase chain reaction

Identifiers

PMID41903454

DOI10.1016/j.psj.2026.106836

PMCPMC13054034

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.