ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
Directed Evolution Improves the Catalytic Efficiency of APEX2-Mediated Proximity-Dependent RNA Labeling.
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
Abstract read
In one paragraphArticle in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
4 authors.
Gang WangCollege of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory For Molecular Sciences, PKU-IDG/McGovern Institute for Brain Research, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Beijing Advanced Center of RNA Biology (BEACON), Peking University, Beijing, China.ORCID https://orcid.org/0009-0003-7799-0196 Yi LiCollege of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory For Molecular Sciences, PKU-IDG/McGovern Institute for Brain Research, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Beijing Advanced Center of RNA Biology (BEACON), Peking University, Beijing, China.
Peiyuan MengCollege of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory For Molecular Sciences, PKU-IDG/McGovern Institute for Brain Research, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Beijing Advanced Center of RNA Biology (BEACON), Peking University, Beijing, China.
Peng ZouCollege of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory For Molecular Sciences, PKU-IDG/McGovern Institute for Brain Research, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Beijing Advanced Center of RNA Biology (BEACON), Peking University, Beijing, China.ORCID https://orcid.org/0000-0002-9798-5242 Funding
Ministry of Science and Technology of the People's Republic of China 2022YFA1304700National Natural Science Foundation of China 32088101
6 · The paper itselfAbstract
Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin-aniline (Btn-An) remains suboptimal. To overcome this limitation, we performed yeast surface display-based directed evolution to enhance APEX2 activity toward Btn-An. The resulting variant,
Indexed as
Ascorbate PeroxidasesDirected Molecular EvolutionDNA-(Apurinic or Apyrimidinic Site) LyaseBiotinEndonucleasesMultifunctional EnzymesRNA, MessengerSaccharomyces cerevisiaeAPEX2 protein, humanAscorbate PeroxidasesBiotinDNA-(Apurinic or Apyrimidinic Site) LyaseEndonucleasesMultifunctional EnzymesRNA, Messenger
Identifiers
PMID41903118
PMCPMC13252616
What OpenQuestion holds
Textmetadata
LicenceCC BY
Read underepoch 390