Evidence map›Paper›PMID 41902218›Full record

ArticleViruses2026

Development and Evaluation of a Novel Relatively Low-Cost Method to Derive HIV-1 Integration Sites and Proviral Sequences.

Samantha R Hardy, Sheila Styrchak, Tim De Meyer, Laurens Lambrechts, Tine Struyve, Basiel Cole, Liesbet Termote, Sherry McLaughlin, James I Mullins, Linos Vandekerckhove and 1 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Samantha R HardyCenter for Global Infectious Disease Research, Seattle Children's Research Institute, University of Washington, Seattle, WA 98109, USA.
Sheila StyrchakCenter for Global Infectious Disease Research, Seattle Children's Research Institute, University of Washington, Seattle, WA 98109, USA.
Tim De MeyerDepartment of Data Analysis and Mathematical Modelling, Ghent University, 9000 Ghent, Belgium.ORCID 0000-0003-2994-9693
Laurens LambrechtsHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, 9000 Ghent, Belgium.
Tine StruyveHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, 9000 Ghent, Belgium.ORCID 0000-0002-9637-9953
Basiel ColeHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, 9000 Ghent, Belgium.
Liesbet TermoteHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, 9000 Ghent, Belgium.
Sherry McLaughlinCenter for Global Infectious Disease Research, Seattle Children's Research Institute, University of Washington, Seattle, WA 98109, USA.
James I MullinsDepartment of Microbiology, University of Washington, Seattle, WA 98109, USA.
Linos VandekerckhoveHIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, 9000 Ghent, Belgium.
Lisa M FrenkelCenter for Global Infectious Disease Research, Seattle Children's Research Institute, University of Washington, Seattle, WA 98109, USA.ORCID 0000-0001-9566-8959

Funding

LOC-IMPAACT Leadership GroupPharmacokinetics and Safety of Remdesivir for Treatment of COVID-19 in Pregnant Women in the USUM1AI068632 · NIAID · SOCIAL AND SCIENTIFIC SYSTEMS, INC. · PI Jennifer Jao, Sharon A Nachman · 2011 to 2026
$278.1M
Statistical and Data Management Center-Pediatric,Adolescent, and Maternal CTGUM1AI068616 · NIAID · HARVARD UNIVERSITY D/B/A HARVARD SCHOOL OF PUBLIC HEALTH · PI Sean Scott Brummel, Marlene Ann Cooper · 2011 to 2026
$155.6M
University of Washington/Fred Hutch Center for AIDS ResearchP30AI027757 · NIAID · UNIVERSITY OF WASHINGTON · PI CONNIE L CELUM · 1988 to 2026
$104.9M
LC - IMPAACT Leadership GroupUM1AI106716 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Grace M Aldrovandi · 2014 to 2026
$47.2M
NIAID NIH HHS P30 AI027757NIAID NIH HHS R01 AI134419-01NIAID NIH HHS UM1 AI068616NIAID NIH HHS UM1AI068616NIAID NIH HHS UM1 AI068632NIAID NIH HHS UM1AI068632NIAID NIH HHS UM1AI106716NICHD NIH HHS HHSN275201800001I
6 · The paper itself

Abstract

In people taking antiretroviral therapy (ART) for HIV infection, the methods to characterize latent and active HIV reservoirs remain costly and labor-intensive. Our objective was to develop a relatively low-cost technique to amplify and sequence the proviruses that persist during ART along with the site in the human genome where each provirus is integrated. We developed a novel HIV-specific Multiple Displacement Amplification (

Indexed as

HIV-1HIV InfectionsNucleic Acid Amplification TechniquesProvirusesVirus IntegrationDNA, ViralHumansDNA, ViralHIV-1integration site (IS)multiple displacement amplification (MDA)provirusREPLI-g MDAwhole-genome amplification (WGA)

Identifiers

PMID41902218
PMCPMC13030101

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.