Evidence map›Paper›PMID 41901763›Full record

ArticlePathogens (Basel, Switzerland)2026

Improving Molecular Detection of Tick-Borne Pathogens in Citizen-Collected Ticks.

Andrea Matucci, Salvatore Scarso, Graziana Da Rold, Federica Obber, Filippo Marzoli, Andrea Ragusa, Fabio Formenti, Davide Treggiari, Antonio Mori, Cristina Mazzi and 10 more

Abstract read
In one paragraph

Article in Pathogens (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Andrea MatucciDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0001-9368-850X
Salvatore ScarsoDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0003-2956-8997
Graziana Da RoldIstituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Centro Specialistico Fauna Selvatica, SCT2-Belluno, 32100 Belluno, Italy.ORCID 0000-0002-4935-0349
Federica ObberIstituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Centro Specialistico Fauna Selvatica, SCT2-Belluno, 32100 Belluno, Italy.ORCID 0000-0002-5459-7880
Filippo MarzoliIstituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Centro Specialistico Fauna Selvatica, SCT2-Belluno, 32100 Belluno, Italy.ORCID 0000-0001-8761-8206
Andrea RagusaDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.
Fabio FormentiDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0003-3203-0872
Davide TreggiariDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.
Antonio MoriDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0003-2845-5931
Cristina MazziClinical Research Unit, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0002-4561-3447
Andrea TedescoDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0001-5097-1890
Pietro SpongaDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.
Giulia BertoliDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0003-4175-1620
Lucia MoroDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0002-6017-1181
Concetta CastillettiDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0001-9819-236X
Carlo Vittorio CitterioIstituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Centro Specialistico Fauna Selvatica, SCT2-Belluno, 32100 Belluno, Italy.ORCID 0000-0002-4482-1295
Dora BuonfrateDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0003-0108-6822
Federico Giovanni GobbiDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.
Francesca PerandinDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0003-3933-3892
Chiara PiubelliDepartment of Infectious, Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Negrar di Valpolicella, 37024 Verona, Italy.ORCID 0000-0002-5739-3795

Funding

Ministero della Salute "Fondi Ricerca Corrente" to IRCCS Sacro Cuore Don Calabria Hospital-L1P7Ministero dell'università e della ricerca Project no. PE00000007, INF-ACT
6 · The paper itself

Abstract

This study aimed primarily to evaluate the performance of two Conformité Européenne-In Vitro Diagnostic (CE-IVD) multiplex real-time PCR (rt-PCR) assays for the molecular identification of tick-borne pathogens (TBPs) of human interest on ticks removed from human skin and collected through a citizen science-based approach. As a secondary objective, the aggregated results were used to describe tick species distribution, developmental stages, and seasonal TBP circulation in 2024 in the considered area. The comparison was conducted on 116 tick samples collected in 2024 voluntarily delivered to a hospital in northeastern Italy. Detected TBPs were further confirmed with in-house-validated PCR methods and, where applicable, resolved to the species level. Clinically relevant pathogen species were identified as single infections or coinfections. Overall, 33.6% of tick samples tested positive for at least one TBP, and 6.9% showed coinfections. Kit B exhibited a higher detection rate for

Indexed as

Molecular Diagnostic TechniquesTick-Borne DiseasesTicksAnimalsBorreliaCitizen ScienceHumansItalyIxodesMultiplex Polymerase Chain ReactionReal-Time Polymerase Chain ReactionRickettsiaSeasonsCE-IVD multiplex real-time PCRcitizen sciencetick-borne pathogenstickszoonoses

Identifiers

PMID41901763
PMCPMC13029150

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.