Evidence map›Paper›PMID 41900452›Full record

ArticleMicroorganisms2026

Advancing HIV Diagnostics: Comparative Evaluation of Multisure HIV-1/2 Rapid Confirmatory Test Against Geenius and Traditional Reference Assays Within a CDC-Aligned Diagnostic Framework.

Ahmed Ismail, Israa M Salameh, Nadin Younes, Parveen B Nizamuddin, Shaden Abunasser, Salma Younes, Sara Abdelmohsen, Mazen N Abouassali, Manal Elshaikh, Ibrahim W Karimeh and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Ahmed IsmailLaboratory Section, Medical Commission Department, Ministry of Public Health, Doha P.O. Box 42, Qatar.ORCID 0000-0003-0102-8155
Israa M SalamehBiomedical Research Center, Qatar University, Doha 2713, Qatar.
Nadin YounesBiomedical Research Center, Qatar University, Doha 2713, Qatar.
Parveen B NizamuddinBiomedical Research Center, Qatar University, Doha 2713, Qatar.
Shaden AbunasserBiomedical Research Center, Qatar University, Doha 2713, Qatar.
Salma YounesBiomedical Research Center, Qatar University, Doha 2713, Qatar.
Sara AbdelmohsenDepartment of Biomedical Science, College of Health Sciences, Qatar University Health Sector, Qatar University, Doha 2713, Qatar.
Mazen N AbouassaliLaboratory Section, Medical Commission Department, Ministry of Public Health, Doha P.O. Box 42, Qatar.
Manal ElshaikhLaboratory Section, Medical Commission Department, Ministry of Public Health, Doha P.O. Box 42, Qatar.
Ibrahim W KarimehLaboratory Section, Medical Commission Department, Ministry of Public Health, Doha P.O. Box 42, Qatar.
Mohammed A IbrahimLaboratory Section, Medical Commission Department, Ministry of Public Health, Doha P.O. Box 42, Qatar.
Mutaz M AliLaboratory Section, Medical Commission Department, Ministry of Public Health, Doha P.O. Box 42, Qatar.
Ibrahim Al ShaarLaboratory Section, Medical Commission Department, Ministry of Public Health, Doha P.O. Box 42, Qatar.
Haris OngMP Biomedicals Asia Pacific Pte. Ltd., 2 Pioneer Place, Singapore 627885, Singapore.ORCID 0009-0005-9336-1140
Çiğdem S ZhmurovDepartment of Molecular Biology and Genetics, Graduate School of Science, Üsküdar University, Istanbul 34662, Türkiye.
Hadi M YassineBiomedical Research Center, Qatar University, Doha 2713, Qatar.ORCID 0000-0001-7592-2788
Laith J Abu-RaddadInfectious Disease Epidemiology Group, Weill Cornell Medicine-Qatar, Cornell University, Qatar Foundation-Education City, Doha 24144, Qatar.ORCID 0000-0003-0790-0506
Houssein AyoubMathematics Program, Department of Mathematics and Statistics, College of Arts and Sciences, Qatar University, Doha 2713, Qatar.ORCID 0000-0003-2512-6657
Gheyath K NasrallahBiomedical Research Center, Qatar University, Doha 2713, Qatar.ORCID 0000-0001-9252-1038

Funding

Qatar University QUCG-CAS-23/24-114QNRF GSRA8-L-1-0501-21022QNRF NPRP13S-0128-200185QRDI ARG01-0524-230321QRDI UREP30-041-3-014
6 · The paper itself

Abstract

Human immunodeficiency virus (HIV) remains a major global health challenge, requiring accurate diagnostic testing for early detection. Chemiluminescent immunoassay screening, particularly the Architect HIV Ag/Ab Combo assay, followed by immunoblot confirmation using INNO-LIA™ has traditionally been used in many diagnostic workflows. To address these limitations, the U.S. Centers for Disease Control and Prevention (CDC) recommends the use of an HIV-1/2 antibody differentiation immunoassay, such as the Geenius HIV-1/2 Supplemental Assay, as part of the confirmatory testing algorithm. This study evaluates the performance of two rapid HIV-1/2 confirmatory assays-the Multisure HIV-1/2 Confirmatory Test and the Bio-Rad Geenius HIV-1/2 Supplemental Assay-within a CDC-aligned diagnostic framework, with the aim of assessing Multisure as a potential alternative differentiation assay. A total of 224 archived serum samples were analyzed, including true positives (

Indexed as

HIV-1/2 differentiationHIV confirmatory assaysHIV diagnostic algorithmrapid diagnostic tests

Identifiers

PMID41900452
PMCPMC13028974

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.