Fungal Diversity Drives Non-Linear Trajectories of Soil Multifunctionality During Alpine Grassland Restoration.

Despite the widely recognized importance of grassland restoration for soil multifunctionality (SMF), its temporal dynamics along the restoration chronosequence and the relative contributions of bacterial and fungal diversity to SMF remain poorly understood, particularly in alpine grasslands. Here, we examined SMF along an alpine grassland restoration chronosequence (1, 5, 7, 13, and 20 years) on the Qinghai-Tibet Plateau. We found that SMF exhibited a pronounced non-linear trajectory, increasing by 39.13% from year 1 to year 7, subsequently declining by 50% and 46.88% at years 13 and 20, respectively, relative to the peak at year 7. Fungal richness varied markedly across the restoration chronosequence, peaking in year 5 with a 16.03% increase relative to year 1, and was positively associated with SMF, whereas bacterial richness showed no significant relationship. Structural equation modeling further confirmed that, along with soil moisture, fungal richness was significantly associated with SMF. Together, our findings highlight fungal diversity as a key driver of SMF during alpine grassland restoration and improve process-based predictions of alpine grassland functioning under ongoing climate change.