Evidence map›Paper›PMID 41899469›Full record

ArticleCurrent issues in molecular biology2026

Exploring the Antimicrobial Potential of a Novel Phage-Derived Lytic Protein Against

Sibongile Mtimka, Kanyane Bridgett Malatji, Patrick Opare Sakyi, Noel David Nogbou, Andrew Munyalo Musyoki, Sipho Mamputha, Lusisizwe Kwezi, Samuel Kojo Kwofie, Ofentse Jacob Pooe, Tsepo Lebiletsa Tsekoa

Abstract read
In one paragraph

Article in Current issues in molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sibongile MtimkaChemicals Cluster, Council for Scientific and Industrial Research (CSIR), Pretoria 0001, South Africa.
Kanyane Bridgett MalatjiChemicals Cluster, Council for Scientific and Industrial Research (CSIR), Pretoria 0001, South Africa.
Patrick Opare SakyiDepartment of Chemical Sciences, University of Energy and Natural Resources, Sunyani P.O. Box 214, Ghana.ORCID 0000-0001-7264-8680
Noel David NogbouDepartment of Microbiology, School of Medicine, Sefako Makgatho Health Sciences University, Pretoria 0204, South Africa.ORCID 0000-0003-0938-0073
Andrew Munyalo MusyokiDepartment of Microbiology, School of Medicine, Sefako Makgatho Health Sciences University, Pretoria 0204, South Africa.ORCID 0000-0002-6577-6155
Sipho MamputhaChemicals Cluster, Council for Scientific and Industrial Research (CSIR), Pretoria 0001, South Africa.ORCID 0009-0004-4941-4674
Lusisizwe KweziChemicals Cluster, Council for Scientific and Industrial Research (CSIR), Pretoria 0001, South Africa.
Samuel Kojo KwofieBiomedical Engineering Department, School of Engineering Sciences, University of Ghana, Legon, Accra Ga488-6615, Ghana.ORCID 0000-0002-1093-1517
Ofentse Jacob PooeDiscipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Durban 4001, South Africa.ORCID 0000-0002-2967-7846
Tsepo Lebiletsa TsekoaChemicals Cluster, Council for Scientific and Industrial Research (CSIR), Pretoria 0001, South Africa.ORCID 0000-0002-5561-5462

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The escalation of bacterial resistance to existing antibiotics represents a growing global health challenge, exacerbated by the widespread misuse of antimicrobial agents. As a result, alternative antibacterial strategies are increasingly being explored, including phage-derived lytic proteins. In this study, we report a preliminary characterisation of a novel phage-derived lytic protein identified through computational screening of bacteriophage genome sequences. A putative open reading frame, designated SM07 (1383 bp), was selected from bacteriophage sequences contributed by the University of KwaZulu-Natal to a global phage repository. The gene was synthesised, sub-cloned into the pET-30b(+) vector with an N-terminal histidine tag, and recombinantly expressed in

Indexed as

antimicrobialbacteriophage therapycatalytic domainendolysinphage-derived proteinPseudomonas aeruginosa

Identifiers

PMID41899469
PMCPMC13025223

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.