Evidence map›Paper›PMID 41898812›Full record

ArticleGenes2026

Genetic Monitoring of a Newly Established Grey Wolf Population in a Peri-Urban Protected Area with First Insights into Wolf-Dog Hybridization in Greece.

Aimilia Ioakeimidou, Yorgos Iliopoulos, Aristotelis Moulistanos, Kerasia Galani, Athanasia Fyta, Eirini Antoniadi, Georgios Bartzokas, Theodoros Kampouris, Caroline Sophie Birkenhain, Gregor Rolshausen and 4 more

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In one paragraph

Article in Genes, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Aimilia IoakeimidouDepartment of Environment, School of the Environment, University of the Aegean, 81100 Mytilene, Greece.
Yorgos IliopoulosCallisto Wildlife and Nature Conservation Society, 54640 Thessaloniki, Greece.ORCID 0000-0001-7136-4002
Aristotelis MoulistanosDepartment of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.ORCID 0000-0001-5678-5060
Kerasia GalaniDepartment of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Athanasia FytaDepartment of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Eirini AntoniadiCallisto Wildlife and Nature Conservation Society, 54640 Thessaloniki, Greece.
Georgios BartzokasCallisto Wildlife and Nature Conservation Society, 54640 Thessaloniki, Greece.
Theodoros KampourisIntermunicipal Stray Animals Care Centre, Schisto Industrial Park, 18863 Perama, Greece.
Caroline Sophie BirkenhainCentre for Wildlife Genetics, Senckenberg Research Institute and Natural History Museum Frankfurt, Clamecystrasse 12, 63571 Gelnhausen, Germany.ORCID 0009-0002-7695-0064
Gregor RolshausenCentre for Wildlife Genetics, Senckenberg Research Institute and Natural History Museum Frankfurt, Clamecystrasse 12, 63571 Gelnhausen, Germany.
Carsten NowakCentre for Wildlife Genetics, Senckenberg Research Institute and Natural History Museum Frankfurt, Clamecystrasse 12, 63571 Gelnhausen, Germany.
Triantafyllos AkriotisDepartment of Environment, School of the Environment, University of the Aegean, 81100 Mytilene, Greece.ORCID 0000-0003-2907-4065
Maria PapandreouManagement Unit of Parnitha and Schinias National Parks and Protected Areas of Saronikos Gulf, Natural Environment and Climate Change Agency (N.E.C.C.A.), 13672 Acharnes, Greece.
Nikoleta KaraiskouDepartment of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

Funding

Greek Wildlife Alliance initiative I have not received a number for this grantLIFE WILD WOLF project LIFE21-NAT-IT-LIFE-WILD-WOLF/101074417
6 · The paper itself

Abstract

BACKGROUND/

objectivesFollowing centuries of systematic eradication, grey wolf (

methodsOne hundred and twenty-four wolf scat samples were genotyped at 20 canine-specific autosomal microsatellite loci and compared to available reference tissue samples from wolves and free-ranging dogs.

resultsA minimum of 31 unique wolf individuals were identified, structured into at least three packs. No wolf-dog hybrids were detected in the study area. To validate the accuracy of the microsatellite analysis, an ancestry informative 93-SNP panel was applied to non-invasive wolf DNA samples from the study area, confirming the absence of hybrids among them. However, a possible wolf-dog hybrid was detected among reference wolf samples collected in Northern Greece, where individuals with atypical morphological traits are observed. The estimated census population size was in accordance with concurrently obtained camera trapping data, while heterozygosity values were low.

conclusionsThis research represents the first systematic effort in Greece to genetically monitor wolves recently established in a protected area. It highlights the need for targeted management strategies based on genetic data to ensure balanced long-term conservation of wolves in peri-urban areas.

Indexed as

Hybridization, GeneticWolvesAnimalsConservation of Natural ResourcesDogsFemaleGenetics, PopulationGenotypeGreeceMicrosatellite RepeatsPolymorphism, Single Nucleotideanthropogenic hybridizationgenetic diversitylarge carnivoresmicrosatellitesnon-invasive sampling

Identifiers

PMID41898812
PMCPMC13026283

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.