Creation of an Engineered Oxygen-Insensitive L-Glutamate Oxidase for the Application of Electrochemical L-Glutamate Sensors.

Mika Hatada, Shouhei Takamatsu, Ryutaro Asano, Kazunori Ikebukuro, Wakako Tsugawa, Koji Sode

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Mika HatadaLampe Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USA.ORCID 0009-0005-8098-9561

Shouhei TakamatsuLampe Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USA.

Ryutaro AsanoDepartment of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan.ORCID 0000-0001-6795-8377

Kazunori IkebukuroDepartment of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan.ORCID 0000-0003-2838-0562

Wakako TsugawaDepartment of Biotechnology and Life Science, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan.ORCID 0000-0001-6299-5267

Koji SodeLampe Joint Department of Biomedical Engineering, The University of North Carolina at Chapel Hill and North Carolina State University, Chapel Hill, NC 27599, USA.ORCID 0000-0002-9833-2091

Funding

The Lampe Joint Department of Biomedical Engineering at the University of North Carolina and Chapel Hill Not Available

6 · The paper itself

Abstract

L-glutamate (L-Glu) is the primary excitatory neurotransmitter in the mammalian central nervous system. Developing a real-time monitoring system is essential to understanding the onset and progression of related conditions. However, the absence of an L-Glu dehydrogenase that is insensitive to oxygen limits the development of oxygen-independent electrochemical enzymatic sensors. Additionally, the most commonly used L-Glu-specific oxidase requires site-specific proteolytic post-translational modifications in specific host microorganisms, which makes protein engineering difficult. To address these issues, L-Glu oxidase derived from

Indexed as

Amino Acid OxidoreductasesBiosensing TechniquesElectrochemical TechniquesGlutamic AcidOxygenProtein EngineeringOxidation-ReductionStreptomycesAmino Acid OxidoreductasesGlutamic AcidL-glutamate oxidaseOxygendye-mediated dehydrogenase activityL-glutamateL-glutamate oxidaseneurotransmitterquasi-direct electron transfertransient open circuit potential

Identifiers

PMID41898688

DOI10.3390/ijms27062831

PMCPMC13026155

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.