Metagenomic Insights into the Effects of Dietary Thymol on the Structure and Function of the Rumen Microbial Community in Beef Steers Consuming Forage.

Emma P Fukuda, Yuan Lu, Emily Fowler, Russell W Jessup, Merritt L Drewery

Abstract read

In one paragraph

Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

5 authors.

Emma P FukudaDepartment of Agricultural Sciences, Texas State University, 601 University Dr., San Marcos, TX 78666, USA.

Yuan LuInstitute for Molecular Life Sciences, Texas State University, 601 University Dr., San Marcos, TX 78666, USA.

Emily FowlerTranslational Health Research Center, Texas State University, 601 University Dr., San Marcos, TX 78666, USA.ORCID 0009-0006-8543-2962

Russell W JessupDepartment of Crop and Soil Sciences, Texas A&M University, 400 Bizzell St., College Station, TX 77843, USA.

Merritt L DreweryDepartment of Agricultural Sciences, Texas State University, 601 University Dr., San Marcos, TX 78666, USA.ORCID 0000-0003-3558-9532

Funding

National Institute of Food and Agriculture 2020-38422-32250National Institute of Food and Agriculture 2021-77040-34881Translational Health Research Center N/a

6 · The paper itself

Abstract

While essential oils are gaining momentum as a strategy to modulate rumen function and potentially reduce enteric methane in cattle, little is known about how their bioactive components, terpenes, affect rumen microbes. Our objective was to evaluate how in vivo doses of thymol affect the structure and function of the rumen microbial community via whole genome shotgun sequencing (WGS). Four beef steers were used in a 4 × 4 Latin square with four 28 d periods. Steers consumed ad libitum forage and received one of four thymol doses (0 [CON], 120 [120-T], 240 [240-T], and 480 [480-T] mg/kg forage intake). Rumen contents were separated into liquid and solid fractions, DNA was extracted, analyzed via WGS, and assessed with orthogonal contrasts. After FDR correction, no taxa were affected by thymol; however, raw

Indexed as

beef cattleessential oilsplant extractsrumen microbiomeshotgun sequencingthymol

Identifiers

PMID41897927

DOI10.3390/ani16060950

PMCPMC13023239

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.