A DPO-Enhanced Gold Nanoparticle-Assisted PCR Assay for Simultaneous Detection of Bovine Viral Diarrhea Virus, Astrovirus, and Rotavirus.

Dongjie Cai, Yulin Lin, Jie Chen, Bin Tian, Qing Liu, Xiaoping Ma, Jiabin Gao, Zhicai Zuo

Abstract read

In one paragraph

Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Dongjie CaiKey Laboratory of Agricultural Animal Diseases and Veterinary Public Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.ORCID 0000-0002-5422-518X

Yulin LinKey Laboratory of Agricultural Animal Diseases and Veterinary Public Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Jie ChenXuanhan County Animal Disease Prevention and Control Center, Dazhou 636150, China.

Bin TianInstitute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Qing LiuKey Laboratory of Agricultural Animal Diseases and Veterinary Public Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Xiaoping MaKey Laboratory of Agricultural Animal Diseases and Veterinary Public Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.ORCID 0000-0002-4950-8441

Jiabin GaoBaicheng Institute of Animal Husbandry, Baicheng 137000, China.

Zhicai ZuoKey Laboratory of Agricultural Animal Diseases and Veterinary Public Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Funding

China Agriculture Research System of MOF and MARA (Beef Cattle/Yak) CARS-37the Sichuan Agricultural University Discipline Development Dual Support Program Special Project 2521993028/2025the Sichuan beef cattle innovation team of National modern agricultural industry technology system SCCXTD-2024-13

6 · The paper itself

Abstract

To develop a rapid detection tool for major bovine diarrheal viruses, Bovine viral diarrhea virus (BVDV), Bovine astrovirus (BAstV), and Bovine rotavirus (BRV), a gold nanoparticle-enhanced dual-priming oligonucleotide (DPO) multiplex PCR method was established. After optimization, the assay showed high sensitivity with detection limits of 4.9 × 10

Indexed as

bovine astrovirusbovine rotavirusbovine viral diarrhea virusdual-priming oligonucleotidenanoparticle-assisted PCR assay

Identifiers

PMID41897891

DOI10.3390/ani16060914

PMCPMC13023237

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.