Development of a Layer-by-Layer Zein/CMCS Microcapsule Platform for Bacteriophage Delivery: A Proof-of-Concept Study Using a Model Phage in Sea Bass.

Weiquan Liang, Tangwu Qiu, Zheng Cheng, Yunqian Sun, Yunyun Zhong, Xueqin Zhang, Le Zhong

Abstract read

In one paragraph

Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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5 · Who and what money

Authors and funding

7 authors.

Weiquan LiangCollege of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.ORCID 0009-0000-0089-6706

Tangwu QiuCollege of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

Zheng ChengCollege of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

Yunqian SunCollege of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

Yunyun ZhongCollege of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

Xueqin ZhangCollege of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

Le ZhongCollege of Light Industry and Food Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.

Funding

State Key Laboratory of Advanced Papermaking and Paper-based Materials 202501the Fundamental Research Funds from the Zhongkai University of Agriculture and Engineering KA25YY17011the Special Project of Graduate Science and Technology Backyard from Zhongkai University of Agriculture and Engineering KA25YY16748

6 · The paper itself

Abstract

Bacteriophages (phages) offer a targeted biocontrol solution, but their direct application is hampered by environmental instability. To address this, we developed a novel, food-grade microcapsule system for phage delivery using layer-by-layer (LbL) self-assembly of zein and carboxymethyl chitosan (CMCS). Lytic phages targeting specific spoilage bacteria were successfully encapsulated via electrostatic interactions. Characterization confirmed the formation of a multilayer structure, driven primarily by hydrogen bonding and electrostatic forces between the wall materials. The microencapsulation markedly enhanced phage stability against thermal (60 °C and 70 °C) and extreme pH (2.0, 12.0) stresses and provided a controlled release profile in a simulated fish exudate. When applied to fresh-cut sea bass (

Indexed as

bacteriophagebiopreservationfresh-cut sea basslayer-by-layermicrocapsule

Identifiers

PMID41897752

DOI10.3390/foods15061032

PMCPMC13024753

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.