Evidence map›Paper›PMID 41897318›Full record

ReviewBiomolecules2026

Key Methodologies in Characterizing the Multi-Scale Structures of Gluten Proteins in Dough: A Comparative Review.

Feifei Su, Yiyuan Zou, Zehua Zhang, Zhiling Tang, Haoran Luo, Fayin Ye, Guohua Zhao

Abstract readReviewComparative Study
In one paragraph

Review in Biomolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Feifei SuCollege of Food Science, Southwest University, 2 Tiansheng Road, Chongqing 400715, China.
Yiyuan ZouCollege of Food Science, Southwest University, 2 Tiansheng Road, Chongqing 400715, China.
Zehua ZhangCollege of Food Science, Southwest University, 2 Tiansheng Road, Chongqing 400715, China.
Zhiling TangCollege of Food Science, Southwest University, 2 Tiansheng Road, Chongqing 400715, China.
Haoran LuoCollege of Food Science, Southwest University, 2 Tiansheng Road, Chongqing 400715, China.
Fayin YeCollege of Food Science, Southwest University, 2 Tiansheng Road, Chongqing 400715, China.ORCID 0000-0001-7726-7596
Guohua ZhaoCollege of Food Science, Southwest University, 2 Tiansheng Road, Chongqing 400715, China.ORCID 0000-0002-2870-0549

Funding

National Natural Science Foundation of China 32572560
6 · The paper itself

Abstract

Gluten proteins are key components in wheat flour that determine the formation of dough and the quality of flour-based products. Upon hydration and mixing, gluten proteins undergo complex structural transformations to form a gluten network, exhibiting a hierarchical multi-scale structure spanning molecular, aggregate, and network scales. Due to the extreme complexity of gluten proteins, accurately characterizing their multi-scale structures remains challenging, requiring the combined application of multiple techniques, which are still relatively limited and thus warrant further exploration. Therefore, this review presents the principles, operational details, and result presentations of current techniques at different structural scales, including electrophoresis, high-performance liquid chromatography, proteomics, Fourier transform infrared spectroscopy, and Fourier transform Raman spectroscopy at the molecular scale; size-exclusion chromatography, asymmetrical flow field-flow fractionation, dynamic light scattering, multi-angle light scattering, differential refractive index, and ultraviolet absorbance at the aggregate scale; and confocal laser scanning microscopy, scanning electron microscopy, confocal Raman microscopy, and two-photon excitation microscopy at the network scale, among others. It further compares the advantages and disadvantages of similar techniques, facilitating their scenario-based selective utilization. Finally, it outlines the ongoing challenges and future perspectives for the development and application of techniques for the multi-scale structural characterization of gluten proteins.

Indexed as

FlourGlutensBreadChromatography, High Pressure LiquidProteomicsSpectroscopy, Fourier Transform InfraredSpectrum Analysis, RamanTriticumGlutensdough formationgluten aggregatesgluten networkin-situ detectionstructural parameters

Identifiers

PMID41897318
PMCPMC13023611

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.