Evidence map›Paper›PMID 41897291›Full record

ArticleBiomolecules2026

Estradiol Reverses Ovariectomy-Induced Small RNA-mRNA Stress Signatures to Restore Neuroendocrine, Synaptic, and Immune Homeostasis in the Hypothalamus.

Muhammad Mubashir, Huan Yang, Xiaohuan Chao, Chunlei Zhang, Jiahao Chen, Yuan Ding, Hongwei Bi, Ziming Wang, Wen Guo, Junhong Fan and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecules, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Muhammad MubashirCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0009-0001-0188-3496
Huan YangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Xiaohuan ChaoCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Chunlei ZhangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Jiahao ChenCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Yuan DingCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Hongwei BiCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Ziming WangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Wen GuoCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Junhong FanCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Mengjun ZhouCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Bo ZhouCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0003-2857-8536

Funding

National Natural Science Foundation of China No. 32172786
6 · The paper itself

Abstract

Loss of ovarian hormones following menopause or ovariectomy is associated with increased anxiety, cognitive impairment, and dysregulation of hypothalamic neuroendocrine pathways. MicroRNAs (miRNAs) and tRNA-derived fragments (tRFs) are emerging classes of small non-coding RNAs that act as post-transcriptional regulators of stress, inflammation, and synaptic function; however, their coordinated involvement in estradiol-mediated hypothalamic regulation remains poorly understood. In this study, adult female mice were assigned to control, estradiol-treated, ovariectomized (OVX), or OVX plus estradiol groups. Anxiety- and cognition-related behaviors were assessed using the open field, Y-maze, and elevated plus maze tests. Circulating estradiol levels and hypothalamic gonadotropin-releasing hormone (GnRH) expression were quantified by ELISA. Hypothalamic mRNA, miRNA, and tRF expression profiles were analyzed by RNA sequencing, followed by differential expression analysis, functional enrichment, integrative network construction, and quantitative real-time PCR validation. Ovariectomy induced anxiety-like behaviors, impaired working memory, reduced estradiol levels, and increased hypothalamic GnRH expression, all of which were reversed by estradiol treatment. Transcriptomic analysis identified 376 differentially expressed miRNAs, 182 differentially expressed tRFs, and 439 differentially expressed mRNAs, enriched in pathways related to stress responses, neuroendocrine regulation, synaptic signaling, metabolic homeostasis, and neuroinflammation. Integrated miRNA-mRNA and tRF-mRNA network analyses revealed several estradiol-responsive miRNAs (including miR-200a-5p, miR-182/183-5p, miR-381-3p, miR-148a-3p, and miR-10 family members) predicting key hub genes such as

Indexed as

EstradiolHypothalamusMicroRNAsNeurosecretory SystemsOvariectomyRNA, MessengerAnimalsFemaleGene Expression RegulationGonadotropin-Releasing HormoneHomeostasisMiceSynapsesEstradiolGonadotropin-Releasing HormoneMicroRNAsRNA, Messengeranxiety-like behaviorestradiolgonadotropin-releasing hormonehypothalamusmicroRNAneuroinflammationovariectomytRFtRNA

Identifiers

PMID41897291
PMCPMC13023533

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.