Evidence map›Paper›PMID 41896338›Full record

ArticleCommunications medicine2026

Emergence of Plasmodium falciparum pfdhps A581G mutation in Southern Senegal under Seasonal Malaria Chemoprevention pressure, 2020-2023.

Mamadou Alpha Diallo, Abdoulaye Tine, Djiby Sow, Aita Sene, Amy Gaye, Yaye D Ndiaye, Jules Gomis, Baba Dieye, Awa B Deme, Mamadou S Yade and 8 more

Abstract read
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In one paragraph

Article in Communications medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Mamadou Alpha DialloInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal. mamadou.diallo@cigass.org.ORCID http://orcid.org/0000-0001-6478-7875
Abdoulaye TineInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Djiby SowInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.ORCID http://orcid.org/0000-0002-6079-3490
Aita SeneInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Amy GayeInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Yaye D NdiayeInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Jules GomisInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Baba DieyeInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Awa B DemeInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Mamadou S YadeInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Bassirou NgomInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Mamane N GarbaInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.ORCID http://orcid.org/0000-0003-1277-7696
Ibrahima M NdiayeInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Khadim DiongueInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Aida Sadikh BadianeInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Mame Cheikh SeckInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Mouhamadou NdiayeInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.
Daouda NdiayeInternational Research and Training Center in Applied Genomic and Health Surveillance (CIGASS), Cheikh Anta Diop University, PO Box 5005, Dakar, Senegal.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMalaria prevention in Senegal includes the use of Seasonal Malaria Chemoprevention with sulfadoxine-pyrimethamine plus amodiaquine. Prolonged use of sulfadoxine-pyrimethamine, however, can select for mutations in the Plasmodium falciparum mutations in pfdhfr and pfdhps genes.

methodsWe analyse dried-blood-spot samples collected during therapeutic-efficacy studies in two SMC districts (Kolda, Kédougou) between 2020 and 2023. Using targeted amplicon deep sequencing we determine the prevalence of pfdhfr and pfdhps mutations by year and site.

resultsThe pfdhfr triple mutant genotype (N51I + C59R + S108N) is near-fixed in the parasite population ( > 90 %). The pfdhps A437G mutation is also common (47-100 %). The K540E mutation is absent in 2020 and appears only sporadically thereafter-14 % of Kédougou isolates in 2021 (1/7), 5.4 % in Kolda and 2.1 % in Kédougou in 2022, and <2 % in both sites in 2023. The A581G mutation emerges in 2022 (8.1 % in Kolda) and rises to 14.9 % in Kolda and 6.5 % in Kédougou by 2023. Mutation frequencies are similar in children ≤ 5 years and 6-10 years.

conclusionsAfter a decade of seasonal chemoprevention, P. falciparum in southern Senegal carries multiple genetic markers associated with resistance to sulfadoxine-pyrimethamine, including a recent increase in the pfdhps A581G mutation to over 10 %. Although the sulfadoxine-pyrimethamine plus amodiaquine regimen remains clinically effective, the growing frequency of pfdhps A581G mutation warrants close monitoring.

Identifiers

PMID41896338
PMCPMC13194753

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.