Evidence map›Paper›PMID 41895274›Full record

ArticleStem cell reports2026

3D Vessels-on-Chip using isogenic hiPSC-derived VSMCs reveal NOTCH3-driven alterations in brain small vessel disease.

Marc Vila Cuenca, Theano Tsikari, Minne N Cerfontaine, James L Gallant, Francijna E van den Hil, Marga J Bouma, Kyra L Dijkstra, Gido Gravesteijn, Antoine A F de Vries, Christine L Mummery and 3 more

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Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Marc Vila CuencaDepartment of Clinical Genetics, Leiden University Medical Center, Leiden, the Netherlands; Department of Anatomy & Embryology, Leiden University Medical Center, Leiden, the Netherlands.
Theano TsikariDepartment of Anatomy & Embryology, Leiden University Medical Center, Leiden, the Netherlands.
Minne N CerfontaineDepartment of Clinical Genetics, Leiden University Medical Center, Leiden, the Netherlands.
James L GallantDepartment of Anatomy & Embryology, Leiden University Medical Center, Leiden, the Netherlands.
Francijna E van den HilDepartment of Anatomy & Embryology, Leiden University Medical Center, Leiden, the Netherlands.
Marga J BoumaLeiden University Medical Center hiPSC Center, Leiden, the Netherlands.
Kyra L DijkstraDepartment of Pathology, Leiden University Medical Center, Leiden, the Netherlands.
Gido GravesteijnDepartment of Clinical Genetics, Leiden University Medical Center, Leiden, the Netherlands.
Antoine A F de VriesDepartment of Cardiology, Leiden University Medical Center, Leiden, the Netherlands.
Christine L MummeryDepartment of Anatomy & Embryology, Leiden University Medical Center, Leiden, the Netherlands.
Julie W RuttenDepartment of Clinical Genetics, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: j.w.rutten@lumc.nl.
Saskia A J Lesnik ObersteinDepartment of Clinical Genetics, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: s.a.m.j.lesnik@lumc.nl.
Valeria V OrlovaDepartment of Anatomy & Embryology, Leiden University Medical Center, Leiden, the Netherlands. Electronic address: v.orlova@lumc.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a hereditary brain small vessel disease caused by pathogenic variants in the NOTCH3 gene, leading to NOTCH3 protein accumulation and degeneration of vascular smooth muscle cells (VSMCs). Here, we developed a CADASIL 3D Vessel-on-Chip model using either primary brain VSMCs or human induced pluripotent stem cell (hiPSC)-derived VSMCs from CADASIL patients and isogenic controls. In 3D co-culture with hiPSC-derived endothelial cells, both primary and hiPSC-derived CADASIL VSMCs exhibited disease-relevant morphological abnormalities, increased NOTCH3 and contractile protein levels, and altered intracellular Ca

Indexed as

BrainCADASILInduced Pluripotent Stem CellsLab-On-A-Chip DevicesMuscle, Smooth, VascularMyocytes, Smooth MuscleReceptor, Notch3Cell DifferentiationCells, CulturedCoculture TechniquesEndothelial CellsHumansMicrophysiological SystemsReceptor, Platelet-Derived Growth Factor betaNOTCH3 protein, humanReceptor, Notch3Receptor, Platelet-Derived Growth Factor betaCADASILdisease modelinghereditary brain small vessel diseasehiPSC-derived vascular smooth muscle cellshiPSC-VSMCsmicrofluidicsNOTCH3SVDvessels on chip

Identifiers

PMID41895274
PMCPMC13083807

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.