Evidence map›Paper›PMID 41893692›Full record

ArticleVeterinary sciences2026

Shotgun Metagenomics Reveals Microbial Diversity, Resistome, and Plasmidome in Dairy Cattle Feces.

Shehla Shehla, Muhammad Kashif Obaid, Sadaf Niaz, Munir Ahmad Khan, Anum Ali Ahmad, Mostafa A Abdel-Maksoud, Abdulaziz Alamri, Salman Alrokayan, Muhammad Shoaib, Sumaira Shams and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Shehla ShehlaDepartment of Zoology, Abdul Wali Khan University, Garden Campus Mardan, Mardan 23200, Pakistan.
Muhammad Kashif ObaidState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
Sadaf NiazDepartment of Zoology, Abdul Wali Khan University, Garden Campus Mardan, Mardan 23200, Pakistan.
Munir Ahmad KhanDepartment of Medicine, Gomal Medical College, Dera Ismail Khan 29050, Pakistan.ORCID 0000-0002-0485-0390
Anum Ali AhmadThe Roslin Institute, The University of Edinburgh, Easter Bush Campus, Edinburgh EH25 9RG, UK.ORCID 0000-0001-6876-4961
Mostafa A Abdel-MaksoudResearch Chair of Biomedical Applications of Nanomaterials, Biochemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Abdulaziz AlamriBiochemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.ORCID 0009-0002-3942-5455
Salman AlrokayanResearch Chair of Biomedical Applications of Nanomaterials, Biochemistry Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Muhammad ShoaibJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0001-8695-1840
Sumaira ShamsDepartment of Zoology, Abdul Wali Khan University, Garden Campus Mardan, Mardan 23200, Pakistan.
Qiaoyun RenState Key Laboratory of Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, College of Veterinary Medicine, Lanzhou University, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.

Funding

King Saud University ORF-RC-2026-2600
6 · The paper itself

Abstract

Fecal microbiota are shaped by upstream digestive processes and reflect the outcome of host-microbe interactions, including the resistant microbial fraction that survives to be excreted. This is particularly crucial for assessing zoonotic risks and environmental contamination, as feces are the primary source of dissemination, which is considered an emerging One Health threat. Therefore, we conducted a pilot study to obtain the exploratory findings regarding the cattle GIT microbial composition, potential resistome, and their transmission drivers, such as plasmids, using metagenomic analysis from different districts in Khyber Pakhtunkhwa (KP) province, Pakistan. For this purpose, a total of 150 fecal samples (50 from each district) of healthy cattle were collected from various farms in Mardan (FC1), Peshawar (FC2), and Dera Ismail Khan (FC3) districts. Total DNA from each sample was extracted, pooled (FC1, FC2, and FC3), and sequenced via the Illumina platform. Bacteria were the highly abundant kingdom, while Pseudomonadota and Bacillota were dominant phyla in all samples.

Indexed as

cattle fecesmetagenomicsmicrobial diversityPakistanplasmidomeresistome

Identifiers

PMID41893692
PMCPMC13030266

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.