Assessment of Fecal Microbiota in Healthy Dogs and Dogs with Cutaneous Mast Cell Tumors Treated with Electrochemotherapy Combined with Gene Electrotransfer of IL-12.

Anja Lisjak, Bruna Correa Lopes, Rachel Pilla, Ana Nemec, Urša Lampreht Tratar, Jan S Suchodolski, Nataša Tozon

Abstract read

In one paragraph

Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Comparative Oncology of Companion Animals.Veterinary sciences · 2026
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Anja LisjakSmall Animal Clinic, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.

Bruna Correa LopesGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0003-1234-599X

Rachel PillaGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0001-7758-2609

Ana NemecSmall Animal Clinic, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.

Urša Lampreht TratarSmall Animal Clinic, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.ORCID 0000-0003-2905-4574

Jan S SuchodolskiGastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.

Nataša TozonSmall Animal Clinic, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia.

Funding

Purina PetCare Research Excellence Fund The microbiome research at the Gastrointestinal Laboratory at Texas A&M University is in part funded through Purina PetCare Research Excellence FundThe Slovenian Research and Innovation Agency P3-0428, P4-0053, P3-0003, and J4-2546

6 · The paper itself

Abstract

Cancer is a major health concern, with its incidence rate continuing to increase. There is growing interest in the microbiota and its role in carcinogenesis, as it significantly influences physiological and pathological processes. Various aspects of the microbiome have been shown to have both anti-tumor and pro-tumor effects. Advances in techniques such as high-throughput DNA sequencing have greatly improved our understanding of microbial populations in the human and canine gut. We aimed to (1) characterize the intestinal microbiota of healthy dogs and dogs with cutaneous mast cell tumors (MCTs), (2) assess changes in the intestinal microbiota of dogs undergoing electrochemotherapy (ECT) combined with gene electrotransfer (GET) of the IL-12 plasmid (IL-12), and (3) explore possible associations with the expression of immune markers Programmed cell death protein 1 (PD-1), Programmed death-ligand 1 (PD-L1), and Granzyme B (GZMB) in MCT tissue. Stool samples were collected from healthy dogs (

Indexed as

canine microbiomedysbiosis indexelectrochemotherapygene electrotransferMCTmetagenomicsshallow shotgun sequencing

Identifiers

PMID41893658

DOI10.3390/vetsci13030241

PMCPMC13030013

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.