Evidence map›Paper›PMID 41892028›Full record

ArticleBiosensors2026

An Immunochromatographic Test Strip and Its Application in Rapid Screening of Pepper Mild Mottle Virus.

Xin Yang, Kelei Han, Wenyao Zhang, Chen Zhang, Rui Fan, Tingtao Chen, Yan Jin, Jiashuo An, Zichen Zhu, Xiaolong Shao and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Xin YangState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Kelei HanInstitute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China.ORCID 0000-0002-4019-9005
Wenyao ZhangState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Chen ZhangState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Rui FanState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Tingtao ChenState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Yan JinState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Jiashuo AnState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Zichen ZhuState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Xiaolong ShaoState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Guoliang QianState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0003-3577-3241
Dankan YanInstitute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Limin WangState Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.

Funding

National Key Research and Development Program of China 2022YFD1400200National Natural Science Foundation of China 32300150
6 · The paper itself

Abstract

The quality and productivity of peppers have been severely impacted by the pepper mild mottle virus (PMMoV). To effectively control the transmission and damage caused by PMMoV, it is crucial to establish a simple, rapid, and field-applicable detection method. In this study, the coat protein of PMMoV was induced expression as an immunogen. After cell fusion and subcloning, a total of 10 hybridoma cell lines that stably secrete the PMMoV monoclonal antibody were screened. Through antibody pairing and screening, using monoclonal antibody 4D7 as the capture antibody and 3B9 as the detection antibody, an immunochromatographic test strip for PMMoV was established. Under ideal conditions, the test strip's color development indicated that its detection limit for the target protein was 1 ng/mL, and the result was obtained in 10 min. The findings of field testing and specific detection demonstrated that this test strip could reliably identify PMMoV and it is capable of distinguishing between different disease samples collected in this study. It is anticipated that this test strip will be able to offer services for PMMoV field detection.

Indexed as

CapsicumChromatography, AffinityTobamovirusAntibodies, MonoclonalAntibodies, ViralCell LinePlant DiseasesPlant LeavesRecombinant ProteinsTime FactorsAntibodies, MonoclonalAntibodies, ViralRecombinant Proteinscoat proteinimmunochromatographic stripmonoclonal antibodiespepper mild mottle virus

Identifiers

PMID41892028
PMCPMC13024521

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.