Evidence map›Paper›PMID 41890729›Full record

ArticleFrontiers in immunology2026

Midgestational injection of highly expanded human CD34+ cells increases lineages of human immune cells and supports thymic development in

Ahlea M Forster, Amanda Ahrens Kress, Matti Kiupel, Joan Cunnick, Dennis A Webster, Jarryd M Campbell, Adrienne L Watson, Ohad Gafni, Daniel F Carlson, Branden S Moriarity and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Ahlea M ForsterDepartment of Animal Science, Iowa State University, Ames, IA, United States.
Amanda Ahrens KressLaboratory Animal Resources, Iowa State University, Ames, IA, United States.
Matti KiupelDepartment of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, United States.
Joan CunnickDepartment of Animal Science, Iowa State University, Ames, IA, United States.
Dennis A WebsterRecombinetics, Inc., New Brighton, MN, United States.
Jarryd M CampbellRecombinetics, Inc., New Brighton, MN, United States.
Adrienne L WatsonRecombinetics, Inc., New Brighton, MN, United States.
Ohad GafniRecombinetics, Inc., New Brighton, MN, United States.
Daniel F CarlsonRecombinetics, Inc., New Brighton, MN, United States.
Branden S MoriarityDepartment of Pediatrics, University of Minnesota, Minneapolis, MN, United States.
Beau R WebberDepartment of Pediatrics, University of Minnesota, Minneapolis, MN, United States.
Brett NapiwockiDepartment of Pediatrics, University of Minnesota, Minneapolis, MN, United States.
Lance DaharshDepartment of Animal Science, Iowa State University, Ames, IA, United States.
Jason W RossDepartment of Animal Science, Iowa State University, Ames, IA, United States.
Mary B SauerLaboratory Animal Resources, Iowa State University, Ames, IA, United States.
Christopher K TuggleDepartment of Animal Science, Iowa State University, Ames, IA, United States.

Funding

Developing second generation SCID pig models: filling the gaps to improve translation of therapeutics in regenerative medicineR24OD028748 · OD · IOWA STATE UNIVERSITY · PI Christopher Tuggle · 2020 to 2026
$4.7M
NIH HHS R24 OD028748
6 · The paper itself

Abstract

Severe combined immunodeficiency (SCID) pigs have become a promising large animal model for biomedical research, offering significant advantages over traditional mouse models due to their anatomical, physiological, and genetic similarities to humans. Humanized SCID pig models can potentially improve preclinical research in areas such as cancer immunotherapies, stem cell therapies, and transplantation methods, yet often lack significant lymphocyte development, including evidence of B cell and myeloid cell development. This work aims to increase the extent of humanization of the SCID pig. CRISPR guide RNAs were successfully developed for the RAG2 and IL2RG genes, and a double-knockout cell line (RAG2-/-IL2RG-/Y, RG) was established. Somatic cell nuclear transfer (SCNT) was then used to create cloned SCID fetuses, which were injected intraperitoneally with

Indexed as

Antigens, CD34DNA-Binding ProteinsInterleukin Receptor Common gamma SubunitSevere Combined ImmunodeficiencyThymus GlandAnimalsAnimals, Genetically ModifiedAnimals, NewbornCell LineageDisease Models, AnimalFemaleHumansNuclear ProteinsSwineAntigens, CD34DNA-Binding ProteinsIL2RG protein, humanInterleukin Receptor Common gamma SubunitNuclear ProteinsRAG2 protein, humanbiomedical model developmenthumanizationSCID-severe combined immunodeficiencyswinetranslational biomedicine

Identifiers

PMID41890729
PMCPMC13012948

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.