Evidence map›Paper›PMID 41890707›Full record

ArticleRegenerative biomaterials2026

Enrichment of colon cancer stem cells via polymeric porous filters with different zeta potentials.

Tzu-Cheng Sung, Ling-Chun Hung, Min Gao, Xuanyu Lin, Manman Yang, Xin Kang, Zeyu Tian, Ting Wang, Jian Gong, Jiandong Pan and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Regenerative biomaterials, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Tzu-Cheng SungState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Ling-Chun HungDepartment of Chemical and Materials Engineering, National Central University, Jhongli, Taoyuan 32001, Taiwan, China.
Min GaoState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Xuanyu LinState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Manman YangState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Xin KangState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Zeyu TianState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Ting WangState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID https://orcid.org/0000-0002-0961-8224
Jian GongDepartment of Clinical Laboratory, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Jiandong PanState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.
Henry Hsin-Chung LeeDepartment of Surgery, Cathay General Hospital, Taipei 106, Taiwan, China.
Akon HiguchiState Key Laboratory of Eye Health, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang 325027, China.ORCID https://orcid.org/0000-0003-2970-8531

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Colorectal cancer is one of the most prevalent malignant tumors worldwide, and cancer-initiating (CI) cells or cancer stem (CS) cells are critical for tumor progression. We purified CI/CS cells from colon cancer cells utilizing a membrane filtration method. We developed membrane filters with different surface charges (zeta potentials) by blending a negatively ionized polymer [poly(vinyl alcohol-itaconic acid), PVI] or a positively ionized polymer [poly(L-lysine), PLL] into poly(lactide-co-glycolic acid) (PLG). Suspensions of HT-29 colon cancer cells and PAT-3 cells (primary colon cancer cells from a colon cancer patient in this research) were permeated through unmodified PLG filters and modified PLG filters blended with and without PVI or PLL. The cells in the filtration and recovering solutions and migrating cells from the filters after filtration were evaluated to identify the cells in each fraction and which filter enriched CI/CS cells. CI/CS cells were evaluated for (a) CD44 and CD133 (CI/CS cell markers) expression by flow cytometry and immunostaining

Indexed as

cancer stem cellcell sortingcolon cancermembrane filtrationpositively charged filter

Identifiers

PMID41890707
PMCPMC13012879

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