Evidence map›Paper›PMID 41890292›Full record

ArticleFrontiers in plant science2026

Enhancement of chilling stress tolerance in ornamental chilli by exogenous application of salicylic acid and ascorbic acid revealed through transcriptomic profiling.

Anam Zahid, Fozia, Muhammad Ramzan, Gao Yike, Muhammad Mohsin, Salim A Ali, Mohammad Fikry, Muhammad Munir, Hesham S Ghazzawy, Hattim M M Makki and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in plant science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. The dual role of polyethylene glycol-simulated drought stress inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Anam ZahidNational Flower Engineering Research Centre, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, College of Landscape Architecture of Beijing Forestry University, Beijing, China.
FoziaNational Flower Engineering Research Centre, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, College of Landscape Architecture of Beijing Forestry University, Beijing, China.
Muhammad RamzanSchool of Forest Sciences, University of Eastern Finland, Joensuu, Finland.
Gao YikeNational Flower Engineering Research Centre, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, College of Landscape Architecture of Beijing Forestry University, Beijing, China.
Muhammad MohsinSchool of Forest Sciences, University of Eastern Finland, Joensuu, Finland.
Salim A AliDate Palm Research Center of Excellence, King Faisal University, Al-Ahsa, Saudi Arabia.
Mohammad FikryDate Palm Research Center of Excellence, King Faisal University, Al-Ahsa, Saudi Arabia.
Muhammad MunirDate Palm Research Center of Excellence, King Faisal University, Al-Ahsa, Saudi Arabia.
Hesham S GhazzawyDate Palm Research Center of Excellence, King Faisal University, Al-Ahsa, Saudi Arabia.
Hattim M M MakkiDate Palm Research Center of Excellence, King Faisal University, Al-Ahsa, Saudi Arabia.
Khaled M RamadanCentral Laboratories, Department of Chemistry, King Faisal University, Al-Ahsa, Saudi Arabia.
Mohamed A A AhmedPlant Production Department (Horticulture - Medicinal and Aromatic Plants), Faculty of Agriculture (Saba Basha), Alexandria Universitya, Alexandri, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ornamental Chillies are valued for their potential to be marketed as potted plants, given their diversity in color and fruit shape. Despite their market value, Chillies are highly susceptible to chilling stress. Salicylic acid (SA) and ascorbic acid (AA) enhance plant cold tolerance by modulating antioxidant defense systems and stress-responsive signaling pathways. However, insufficient information exists on the overall gene expression induced by the combined foliar application of SA and AA under chilling stress in Chillies. In this study, RNA sequencing was utilized to elucidate the molecular mechanisms of combined SA+AA under chilling stress. A comprehensive transcriptomic analysis following exogenous treatment highlighted the impact of 2 mM SA and 2 mM AA on genes contributing to fundamental Chilling stress adaptation. The combined foliar application of SA+AA significantly reduced ROS accumulation by 48% in V1 and 54% in V2 compared with the control. Similarly, MDA content decreased to 3% in V1 relative to 9% and 8% in the control and V2, respectively, indicating reduced oxidative damage and enhanced cellular stability under chilling stress. There were 48346 gene transcripts, and within the sets, 210 genes were differently expressed (DEGs) following V1 treatment and 3933 genes following V2 treatment by SA and AA compared with the control. The cultivar WIZ-21 contains 123 Upstream and 77 downstream DEGs, and cultivar Golden Heart has 44 Upstream and 3979 downstream DEGs. Transcriptome analysis identified 48,346 expressed transcripts, of which a subset met predefined statistical thresholds and were classified as differentially expressed genes (DEGs). Six representative DEGs were selected for RT-qPCR validation of the RNA-seq results in both cultivars. The combined foliar treatment of SA and AA also stimulated the hormonal signaling in cytoplasm, and response to stress-related genes, such as COBRA, MYB14, MYC, GRP, ANK, and ERF proteins. The present investigation, therefore, pinpointed key genes that exhibited altered expression patterns in treated Chillies exposed to chilling stress, which were associated with hormone signaling and metabolism, redox, cellulose synthase-like proteins, and stress defense.

Indexed as

ANKascorbic acidCapsicum annuumcobraERFRNA-Seqsalicylic acid

Identifiers

PMID41890292
PMCPMC13014384

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.