Evidence map›Paper›PMID 41890057›Full record

ArticlebioRxiv : the preprint server for biology2026

Ribosome Molecular Aging Shapes Translation Dynamics.

Jordy F Botello, Lifei Jiang, Peter J Metzger, Troy J Comi, Aya A Abu-Alfa, Qiwei Yu, Margaret S Ebert, Minkyu Lee, Lennard W Wiesner, Maya Butani and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jordy F BotelloDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0002-0163-0595
Lifei JiangDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0009-0007-7291-8311
Peter J MetzgerDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0001-5172-9174
Troy J ComiOmenn-Darling Bioengineering Institute, Princeton University, Princeton NJ, 08544, USA.ORCID 0000-0002-3215-4026
Aya A Abu-AlfaDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0009-0003-8569-2164
Qiwei YuLewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 08544, USA.ORCID 0000-0003-0610-3484
Margaret S EbertOmenn-Darling Bioengineering Institute, Princeton University, Princeton NJ, 08544, USA.ORCID 0000-0002-9122-7854
Minkyu LeeDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.
Lennard W WiesnerDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0001-7161-4358
Maya ButaniDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0009-0005-2713-3019
Claire J WeaverDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0001-6386-5341
Andrej KošmrljDepartment of Mechanical and Aerospace Engineering, Princeton, NJ 08544, USA.ORCID 0000-0001-6137-9200
Ileana M CristeaDepartment of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.ORCID 0000-0002-6533-2458
Clifford P BrangwynneOmenn-Darling Bioengineering Institute, Princeton University, Princeton NJ, 08544, USA.

Funding

Dynamic virus-driven remodeling of ER-mitochondria contactsR01AI174515 · NIAID · PRINCETON UNIVERSITY · PI CRISTEA, ILEANA M. · 2022 to 2025
$2.2M
NIAID NIH HHS R01 AI174515
6 · The paper itself

Abstract

Cellular homeostasis relies on continual renewal of cellular components, yet some complexes like ribosomes persist for long periods, raising the question of whether extended molecular age impacts functional fidelity. Here, we introduce a spatiotemporal mapping strategy to resolve biomolecular life stages, and show that intracellular ribosome aging alters translational dynamics at specific transcripts. Molecularly aged ribosomes exhibit impaired elongation at basic amino acid-rich sequences, leading to increased pausing, premature termination, and ribosome collisions. By profiling ribosomal RNA modifications, we find that molecular aging increases the collision propensity of specific ribosome subpopulations. Consistent with our findings, enrichment of aged ribosomes in cells amplifies molecular age-dependent translation defects.

Indexed as

agingchemogeneticsepitranscriptomicsorganelle proteomicsPROTACspulse chaseribosomeribosome profilingtranslation

Identifiers

PMID41890057
PMCPMC13014177

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.