Evidence map›Paper›PMID 41889465›Full record

ReviewChemical & biomedical imaging2026

The Fantastic Single-Molecule Techniques.

Huang Tang, Shuting Liu, Chenyue Kang, Xiang Wang, Xi Zhang, Kun Li, Gege Duan, Zheng Li, Boyang Hua, Xing-Hua Xia

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Chemical & biomedical imaging, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Huang TangSchool of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Shuting LiuSchool of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Chenyue KangSchool of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Xiang WangSchool of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Xi ZhangSchool of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Kun LiSchool of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Gege DuanSchool of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Zheng LiDepartment of Materials Science and Engineering, College of Chemistry and Materials Science, Jinan University, Guangzhou 511443, China.ORCID https://orcid.org/0000-0001-9066-5791
Boyang HuaSchool of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.ORCID https://orcid.org/0009-0001-6488-6096
Xing-Hua XiaSchool of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.ORCID https://orcid.org/0000-0001-9831-4048

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the past 50 years, single-molecule techniques have been rapidly developed and widely applied in numerous fields of biology, offering insights that conventional biochemical assays cannot discover. In this review, to help fully appreciate the effectiveness of single-molecule methods, we systematically summarize the various advantages of performing biochemical assays at the single-molecule level. To demonstrate that this strategy is not limited to the few representative cases discussed here, we propose a single-molecule polysome profiling technique, taking inspiration from previous examples. Finally, we point out a possibility in the future of unifying different biochemical assays on the platform of single-molecule microscopy, which will reduce the cost of laboratory instrumentation and inevitably promote the adoptability and automation of a variety of biochemical and biophysical methods.

Indexed as

biochemical and biophysical assaysbiomarker detectionbiomolecular interactionselectrochemiluminescencefluorescenceplasmonic scatteringsequencing technologiessingle-molecule techniquessuper-resolution chemical imaging

Identifiers

PMID41889465
PMCPMC13014335

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.