Evidence map›Paper›PMID 41886458›Full record

ArticleSTAR protocols2026

Protocol to isolate, expand, and activate human type 2 innate lymphoid cells to study immune checkpoint function.

Cecilia Ciancaglini, Silvia Santopolo, Paola Vacca, Lorenzo Moretta, Linda Quatrini

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Cecilia CiancagliniIRCCS Bambino Gesù Children's Hospital, Innate Lymphoid Cell Unit, Rome, Italy. Electronic address: cecilia.ciancaglini@opbg.net.
Silvia SantopoloIRCCS Bambino Gesù Children's Hospital, Innate Lymphoid Cell Unit, Rome, Italy.
Paola VaccaIRCCS Bambino Gesù Children's Hospital, Innate Lymphoid Cell Unit, Rome, Italy.
Lorenzo MorettaIRCCS Bambino Gesù Children's Hospital, Tumor Immunology Unit, Rome, Italy.
Linda QuatriniIRCCS Bambino Gesù Children's Hospital, Innate Lymphoid Cell Unit, Rome, Italy. Electronic address: linda.quatrini@opbg.net.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Type 2 innate lymphoid cells (ILC2s) are a subset of innate lymphoid cells involved in allergic diseases, tissue repair, and tumor immunity. Here, we present a protocol to study the function of immune checkpoint receptors on ILC2s in vitro using plate-bound recombinant ligands. We describe steps for the enrichment, expansion, and purification of human ILC2s from healthy donors' peripheral blood. For complete details on the use and execution of this protocol, please refer to Ciancaglini et al.

Indexed as

Cell Culture TechniquesCell SeparationImmune Checkpoint ProteinsImmunity, InnateLymphocytesFlow CytometryHumansImmune Checkpoint ProteinsCancerCell BiologyCell cultureCell isolationFlow CytometryImmunology

Identifiers

PMID41886458
PMCPMC13053985

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.