ArticleMolecular biology reports2026
Transient heat stress irreversibly disrupts molecular signals, cellular and developmental competence of riverine buffaloes (Bubalus bubalis) oocytes and early embryos in a stage-specific manner.
Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA slight, transient increase in heat shock induces stage-specific detrimental effect on reproductive performance of riverine buffaloes (Bubalus bubalis). The vulnerability of oocytes and early embryos, as well as their ability to recover are both restricted and stage-dependent, consequently form major basis for reproduction failure during heat stress.
objectiveThe current study aimed to identify the most sensitive and susceptible stage of oocytes and early embryos against short thermal stress which hampers the reproduction significantly, and intent to unravel the underlying molecular signals responsible for the poor cellular growth and stunted embryonic development. MATERIAL AND
methodSix different in vitro experimental groups of oocytes and early embryos were exposed to 40ºC for 4 h during different stages of in vitro maturation (IVM) and in-vitro fertilization (IVF), as well as in vitro culture (IVC) of early embryos to assess the stage dependent effect of heat stress on buffalo oocytes and early embryos.
resultThe most detrimental effect of heat stress was observed in group 2 with sharp reduction in morula to blastocysts transition rate (P < 0.05). The underlying molecular signals of HSP8, MnSOD and SIRT3 genes were significantly increased from mid-IVM oocytes to 4-cell embryo. Disruption of molecular signal network of genes viz; eIF1A, U2AF, BAX, BCL2, CASPASE9, TEAD4, CDX2, ZO-1, ETS2 and NKB1 was implicated in cellular damage, inconsistent and embryonic development, consequently leading to formation of poor quality of blastocysts with reduced inner cell mass (ICM) and trophectoderm (TE) cells in the affected groups.
conclusionThe current study provides insight into the molecular and cellular effects of short thermal stress on buffaloes oocytes and early embryos, demonstrating development potential and identifying buffalo oocytes and pre-morulae embryos as the most vulnerable stages.
Indexed as
Identifiers
41886158What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.