Evidence map›Paper›PMID 41885417›Full record

ArticleJournal of microbiology & biology education2026

Delivering a multi-color, cell-based CRISPR experience to low-resource classrooms.

Andrew J Walters, Alexander T Lyons, Andrew A Williams, Drew Porter, Clark Riddle, Amanda Moravek, Makail Lunt, Julissa van Renselaar, David Hall, Tyson Barnes and 5 more

Abstract read
In one paragraph

Article in Journal of microbiology & biology education, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Andrew J Walters *Department of Chemistry and Biochemistry, Utah State University, Logan, Utah, USA.ORCID 0000-0002-1302-1738
Alexander T Lyons *Department of Chemistry and Biochemistry, Utah State University, Logan, Utah, USA.ORCID 0009-0006-5128-5757
Andrew A Williams *Department of Chemistry and Biochemistry, Utah State University, Logan, Utah, USA.ORCID 0009-0006-4425-0046
Drew PorterDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
Clark RiddleDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
Amanda MoravekDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
Makail LuntDepartment of Chemistry and Biochemistry, Utah State University, Logan, Utah, USA.
Julissa van RenselaarDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
David HallDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
Tyson BarnesDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
Anna ScribnerDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
Canyon BrysonDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
Ronald SimsDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
Charles MillerDepartment of Biological Engineering, Utah State University, Logan, Utah, USA.
Ryan N JacksonDepartment of Chemistry and Biochemistry, Utah State University, Logan, Utah, USA.ORCID 0000-0001-7403-3794

Funding

Determining the Structure and Function of Newly Discovered Immune SystemsR35GM138080 · NIGMS · UTAH STATE UNIVERSITY · PI Ryan Neal Jackson · 2020 to 2026
$2.8M
NIGMS NIH HHS R35 GM138080
6 · The paper itself

Abstract

CRISPR-based biotechnologies are widely used to diagnose and treat disease, engineer new crops, and examine gene function. The power, simplicity, and effectiveness of CRISPR tools ensure their place as a major biotechnological tool for advancing agriculture, science, and medicine. Thus, education on CRISPR system biology, function, and technology is needed to empower the public regarding the benefits and limitations associated with CRISPR technologies. However, high costs and infrastructure requirements prevent low-resource classrooms from accessing CRISPR-based educational experiences. To address this need, we developed a multicolor, cell-based experience that can be deployed in high school or undergraduate classrooms to educate students about CRISPR biology and biotechnologies. The minimal resource requirements and simplicity of the assay overcome barriers posed by other CRISPR educational tools, providing hands-on access to CRISPR-based experiences for previously unserved and underserved high school and undergraduate students.

Indexed as

biotechnologyCRISPReducationfluorescent proteinsimmune systemslow-resourcemicrobiologyrecombinant-protein productiontransformation

Identifiers

PMID41885417
PMCPMC13130985

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.