Evidence map›Paper›PMID 41885409›Full record

ArticleJournal of extracellular vesicles2026

Hyaluronic Acid Decoration Facilitates CD44-Mediated Targeting and Alters Protein Corona Formation of Extracellular Vesicles.

Heikki Kyykallio, Elena Scurti, Martina Hanzlíková, Kirsti Härkönen, Tuovi Salo, Veera Keinonen, Janne Capra, Tapani Viitala, Jaakko Teppo, Tatu Lajunen and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of extracellular vesicles, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Heikki KyykallioInstitute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland.ORCID https://orcid.org/0000-0002-8455-876X
Elena ScurtiDrug Research Program and Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.ORCID https://orcid.org/0000-0002-9634-1816
Martina HanzlíkováDrug Research Program and Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.ORCID https://orcid.org/0000-0002-5022-0103
Kirsti HärkönenDepartment of Technical Physics, Faculty of Science, Forestry and Technology, University of Eastern Finland, Kuopio, Finland.ORCID https://orcid.org/0009-0003-0526-7935
Tuovi SaloInstitute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland.
Veera KeinonenInstitute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland.
Janne CapraInstitute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland.ORCID https://orcid.org/0000-0001-8446-2357
Tapani ViitalaDrug Research Program and Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.ORCID https://orcid.org/0000-0001-9074-9450
Jaakko TeppoDrug Research Program and Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki, Finland.ORCID https://orcid.org/0000-0003-1022-5572
Tatu LajunenSchool of Pharmacy, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland.
Kirsi RillaInstitute of Biomedicine, School of Medicine, Faculty of Health Sciences, University of Eastern Finland, Kuopio, Finland.ORCID https://orcid.org/0000-0002-7862-5727

Funding

Business Finland 3898/31/2024Helsingin YliopistoItä-Suomen YliopistoResearch Council of FinlandSuomen KulttuurirahastoThe Finnish Research Impact FoundationThe Savo Cancer Fund
6 · The paper itself

Abstract

Cell recognition and uptake of extracellular vesicles (EVs) is mediated by a variety of surface molecules. Growing interest has recently been drawn towards glycan structures on EVs. Hyaluronic acid (HA) is a negatively charged glycosaminoglycan that can decorate the surface of EVs from different origins. HA is a ligand for adhesion receptor CD44, which is overexpressed in various cancers and inflammatory diseases. Although HA has been utilised as a surface decoration to improve CD44-mediated targeting of synthetic nanoparticles and EVs, the role of CD44 in the uptake of EVs is not well known. To assess the importance of CD44 in the interactions and endocytosis of HA-decorated EVs, uptake of HA-decorated and nondecorated EVs into CD44-expressing and -deficient cells was investigated using microscopic methods. The uptake of HA-decorated EVs was significantly increased into cells that expressed CD44, but no differences in endocytosis mechanisms were found. Additionally, the formation of plasma-derived protein corona in HA-decorated and nondecorated EVs was investigated using multi-parametric surface plasmon resonance and mass spectrometry. HA-decoration was found to cause a formation of thicker corona and enrichment of plasma-derived protein corona components. These findings highlight the role of HA in enhancing CD44-mediated EV targeting and modulating the composition of protein corona.

Indexed as

Extracellular VesiclesHyaluronan ReceptorsHyaluronic AcidProtein CoronaAnimalsEndocytosisHumansCD44 protein, humanHyaluronan ReceptorsHyaluronic AcidProtein Coronabiomolecular coronaCD44extracellular vesicleglycanhyaluronic acidprotein corona

Identifiers

PMID41885409
PMCPMC13140515

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.