Evidence map›Paper›PMID 41884841›Full record

ArticleFrontiers in immunology2026

Comprehensive methylome and transcriptome profiling reveals specific biomarkers for bovine viral diarrhea virus persistent infection in calves.

Jiahao Wang, Siqian Chen, Wanyi Lai, Xiao Feng, Qingyao Zhao, Siyuan Mi, Chuang Xu, Tong Qin, Jie Cao, Ying Yu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Jiahao WangNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Siqian ChenNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Wanyi LaiNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Xiao FengNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Qingyao ZhaoNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Siyuan MiNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Chuang XuCollege of Veterinary Medicine, China Agricultural University, Beijing, China.
Tong QinKey Laboratory of Animal Biosafety Risk Prevention and Control (North) & Key Laboratory of Veterinary Biological Products and Chemical Drugs of MARA, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
Jie CaoCollege of Veterinary Medicine, China Agricultural University, Beijing, China.
Ying YuNational Engineering Laboratory for Animal Breeding, State Key Laboratory of Animal Biotech Breeding, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Persistently infected (PI) calves resulting from maternal Bovine viral diarrhea virus (BVDV) infection during early gestation are the main source of viral transmission and pose a serious threat to the sustainable production of herds. PI cattle appear clinically normal, elucidating the molecular-level alterations is critical for understanding their specific characteristics. Moreover, the identification of candidate biomarkers for diagnosing PI cattle will provide valuable insights to support effective strategies for the control and eventual eradication of BVDV. At the same time, few studies have focused on the mothers of PI calves (Trojan dam). Methods: We integrated RNA-seq and reduced representation bisulfite sequencing (RRBS) to characterize the molecular features of PI calves and Trojan dams and identified DNA methylation biomarkers in PI calves. Results: In this study, we found that both B cell and T cell receptor signaling pathways were less active in PI calves, along with a significantly reduced B cell proportion compared to normal calves. By integrating transcriptome and methylation data, we found that the elevated expression of Conclusion: Our study revealed that the immune system of PI cattle is already compromised and identified a novel candidate diagnostic biomarker, providing a new perspective for the detection of PI cattle. In addition, we reveal that prenatal BVDV exposure induces persistent DNA methylation alterations in Trojan dams.

Indexed as

Bovine Virus Diarrhea-Mucosal DiseaseDiarrhea Viruses, Bovine ViralDNA MethylationEpigenomePersistent InfectionTranscriptomeAnimalsBiomarkersCattleFemaleGene Expression ProfilingPregnancyBiomarkersbiomarkerbovine viral diarrhea virusDNA methylationpersistently infectedtrojan dam

Identifiers

PMID41884841
PMCPMC13008631

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.