Evidence map›Paper›PMID 41883010›Full record

ArticleProteomics2026

The N-Glycome to Differentiate Mesenchymal Stem Cells Upon Chondrogenic Differentiation, Dedifferentiation, and Senescence.

Houda Montacir, Karina Biskup, Michael Sittinger, Rudolf Tauber, Véronique Blanchard

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proteomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Houda MontacirCorporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Karina BiskupMedical School Berlin, Department of Human Medicine, Berlin, Germany.
Michael SittingerCorporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Tissue Engineering Laboratory & Berlin-Brandenburg Center for Regenerative Therapies, Dept. of Rheumatology and Clinical Immunology, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Rudolf TauberCorporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Véronique BlanchardCorporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Institute of Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.

Funding

Investitionsbank Berlin and the European Regional Development Fund 10147244and10147246
6 · The paper itself

Abstract

Mesenchymal stem cells (MSCs) are adult stem cells able to self-renewal or differentiation into different cell types, including chondrocytes. N-Glycans are post-translational modifications of glycoproteins that contribute to vital cell functions. In this work, we examined the cell surface N-glycome of human MSCs isolated from bone marrow to identify biomarkers for chondrogenic differentiation. In addition, we investigated for the first time the N-glycome of MSCs upon dedifferentiation and aging. Cell surface glycoproteins were released by tryptic digestion, then N-glycans were enzymatically cleaved, purified, permethylated, and analyzed by MALDI-TOF-MS combined with exoglycosidase digestions. We were able to detect 68 signals, comprising paucimannose, high-mannose, hybrid, and complex-type N-glycans, as well as structures containing polylactosamine motifs. A statistically significant decrease in antennarity and galactosylation, accompanied by an increase in sialylation, was observed during chondrogenic differentiation. Comparison of MSCs at passage 3 and passage 8 revealed increased levels of paucimannosylation, hybrid-type glycans, and sialylation, together with decreased biantennarity, bigalactosylation, and core-fucosylated N-glycans. Dedifferentiated MSCs exhibited a stem cell-like N-glycosylation profile, although statistically significant differences were still detected. These data show that the N-glycosylation profile of MSCs may serve as an indicator of the differentiation stage, dedifferentiation, and aging for the quality control of MSCs.

Indexed as

Cell DedifferentiationCell DifferentiationCellular SenescenceChondrocytesChondrogenesisGlycoproteinsMesenchymal Stem CellsPolysaccharidesCells, CulturedGlycomicsGlycosylationHumansSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationGlycoproteinsPolysaccharidesagingchondrogenic differentiationdedifferentiationglycomicsMALDI‐TOFmesenchymal stem cellsN‐glycanspermethylation

Identifiers

PMID41883010
PMCPMC13327702

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.