ArticleBMC plant biology2026
A single-cell transcriptome atlas reveals the transcriptionally earliest state in rice callus.
Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe cell fate transition of pluripotent callus from somatic cells plays an important role in plant development studies and crop genetic improvement. Embryonic callus is a group of totipotent cells derived from the founder cells responsible for the initiation of callus. However, our understanding of the initial cellular states and regulatory factors of cell fate transition during callus development remains quite limited.
resultsSingle-cell RNA sequencing was employed to generate a single-cell transcriptome atlas of the rice (Oryza sativa L.) embryonic and differentiated callus. We found that the callus founder cell represented the transcriptionally earliest state of embryonic callus, which could transit into the root meristem niche, a process associated with starch degradation, lipid transport, energy production and transfer, plant hormones and flavonoid metabolism. The shoot meristematic cell represented the transcriptionally earliest state of differentiated callus, and these shoot meristematic cells differentiated into mesophyll precursor cells via vascular parenchyma cells, a process associated with chromatin-related epigenetic regulation, enzymatic reactions, signal transduction, metabolic activity and photosynthesis. The expression patterns of the genes involved in plant hormones, and WUSCHEL RELATED HOMEOBOX (WOX) family gene DWARF TILLER1 (OsDWT1) across distinct cell populations suggested that gibberellin (GA) signaling and OsDWT1 were associated with the callus founder cell fate transition.
conclusionsOur results revealed that the callus founder cell and shoot meristematic cell represent the transcriptionally earliest states of embryonic callus and differentiated callus in rice, respectively. This study provides preliminary resources for understanding the development of callus cells and offers clues for improving plant genetic transformation efficiency in recalcitrant rice varieties by regulating cell fate transition.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.