Evidence map›Paper›PMID 41882526›Full record

ArticleBMC genomics2026

Chromosome-scale genome assembly and developmental transcriptome of Aedes triseriatus, vector of La Crosse virus.

Erika Nishiduka, Thomas Hill, Brian Bonilla, Lilian Caesar, Paola Valenzuela Leon, Osvaldo Marinotti, Eric Calvo

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Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Erika NishidukaLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, 20852, USA.ORCID http://orcid.org/0000-0002-4091-1170
Thomas HillIntegrated Data Sciences Section, Research Technology Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID http://orcid.org/0000-0002-4661-6391
Brian BonillaLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, 20852, USA.ORCID http://orcid.org/0000-0002-4067-7476
Lilian CaesarDepartment of Biology, Indiana University, Bloomington, IN, USA.ORCID http://orcid.org/0000-0002-9527-9125
Paola Valenzuela LeonLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, 20852, USA.ORCID http://orcid.org/0000-0002-5740-5178
Osvaldo MarinottiDepartment of Biology, Indiana University, Bloomington, IN, USA.ORCID http://orcid.org/0000-0002-7173-7160
Eric CalvoLaboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, 20852, USA. ecalvo@niaid.nih.gov.ORCID http://orcid.org/0000-0001-7880-2730

Funding

NHLBI NIH HHS AI001246
6 · The paper itself

Abstract

backgroundAedes triseriatus is an endemic North American mosquito and the primary vector of La Crosse virus, the etiologic agent of La Crosse encephalitis. This neuroinvasive disease is the leading cause of arboviral encephalitis in the United States and disproportionately affects pediatric populations. Despite its medical importance, the genomic architecture and vectorial capacity of A. triseriatus remain incompletely understood.

resultsWe generated a chromosome-scale genome assembly and developmental transcriptome for A. triseriatus using an integrated approach combining PacBio long-read sequencing, Hi-C scaffolding, and Illumina sequencing technologies. The final assembly spans approximately 2.4 Gb, representing the largest mosquito genome reported to date, and consists of chromosome-sized scaffolds supported by Hi-C data and manual curation. Transcriptome profiling across larvae, pupae, and adult males and females revealed dynamic, stage-specific gene expression patterns associated with distinct physiological processes.

conclusionsThese genomic and transcriptomic resources provide a comprehensive foundation for investigating the molecular basis of La Crosse virus transmission, mosquito development, and vector–host interactions in A. triseriatus. The chromosome-scale assembly establishes an essential framework for future functional, evolutionary, and vector biology studies in this medically important species.

Indexed as

AedesGenome, InsectLa Crosse virusMosquito VectorsTranscriptomeAnimalsFemaleGene Expression ProfilingGenomicsMaleBlood-feeding arthropodsEncephalitisFunctional genomicsMosquitoVector-borne diseases

Identifiers

PMID41882526
PMCPMC13137584

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.