Evidence map›Paper›PMID 41882187›Full record

ArticleScientific reports2026

PEO-sheathed liquid jets increase sample delivery stability for serial femtosecond X-ray crystallography.

Mohammad Vakili, Saša Bajt, Johan Bielecki, Sabine Botha, Claire Cerniglia, Henry N Chapman, Carsten Deiter, Raphael de Wijn, Katerina Dörner, Juncheng E and 23 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

33 authors.

Mohammad VakiliEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany. mohammad.vakili@xfel.eu.
Saša BajtCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607, Hamburg, Germany.
Johan BieleckiEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Sabine BothaBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Claire CernigliaBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Henry N ChapmanCenter for Free-Electron Laser Science CFEL, Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, 22607, Hamburg, Germany.
Carsten DeiterEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Raphael de WijnEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Katerina DörnerEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Juncheng EEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Petra FrommeBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Raimund FrommeBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Alfonso M Gañán CalvoDepartamento de Ingeniería Aeroespacial y Mecánica de Fluidos, Universidad de Sevilla, E-41092, Sevilla, Spain.
Huijong HanEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Phan Bao Ngoc HuynhBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Chan KimEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Marco KloosEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Faisal H M KouaEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Romain LetrunEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
José Domingo Meza-AguilarBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
José M MontaneroDepto. de Ingeniería Mecánica, Energética y de los Materiales and Instituto de Computación Científica Avanzada (ICCAEx), Universidad de Extremadura, E-06006, Badajoz, Spain.
Katherine MorinBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Tomas PopelarEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Alejandro Rubio GonzálezDepto. de Ingeniería Mecánica, Energética y de los Materiales and Instituto de Computación Científica Avanzada (ICCAEx), Universidad de Extremadura, E-06006, Badajoz, Spain.
Christina SchmidtEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Sunidhi SharmaBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Egor SobolevEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Jui-Tung TsengBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Oleksii TurkotEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Agnieszka WronaEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
Jay-How YangBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Gayathri YogaganeshanBiodesign Center for Applied Structural Discovery, Arizona State University, AZ 85287-5001, Tempe, USA.
Richard BeanEuropean XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Viscoelastic jets can be generated by the polyethylene oxide (PEO) sheathing of an aqueous solution using double-flow focusing nozzles (DFFNs) and represent an efficient method to deliver samples that are dispersed in low and medium-viscosity liquids for X-ray diffractive imaging experiments. Due to their micrometre diameter and millimetre length, such jets can be used for pump-probe serial femtosecond crystallography (SFX) in order to access a timescale of a few tens of microseconds. This range is in between the previously achievable ranges accessible at XFELs (picoseconds-to-microsecond time delays) and synchrotrons (a few hundred µs to millisecond delays), respectively. Here, we demonstrate their effectiveness to deliver protein microcrystals (lysozyme and photosystem II) in buffer compositions of various viscosities for SFX and explore capabilities of triple-flow focusing nozzles (TFFNs) that incorporate PEO-sheathing to control challenging-to-jet viscous buffers for time-resolved diffusive mixing experiments.

Identifiers

PMID41882187
PMCPMC13031959

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.