Evidence map›Paper›PMID 41880513›Full record

ArticleScience advances2026

PRISM: A unified platform for phage isolation and characterization from single-droplet microenvironments.

Han Zhang, Justin Boeckman, Rohit Gupte, Ashlee Prejean, Jonathan Miller, Alexandra Rodier, Paul de Figueiredo, Mei Liu, Jason Gill, Arum Han

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Han ZhangDepartment of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0002-9991-9409
Justin BoeckmanCenter for Phage Technology, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0002-0022-1474
Rohit GupteDepartment of Biomedical Engineering, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0003-3875-8442
Ashlee PrejeanCenter for Phage Technology, Texas A&M University, College Station, TX 77843, USA.ORCID 0009-0001-7394-426X
Jonathan MillerCenter for Phage Technology, Texas A&M University, College Station, TX 77843, USA.
Alexandra RodierDepartment of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0001-5247-7909
Paul de FigueiredoChristopher S. Bond Life Science Center, University of Missouri, Columbia, MO 65211, USA.ORCID 0000-0002-3878-5064
Mei LiuCenter for Phage Technology, Texas A&M University, College Station, TX 77843, USA.
Jason GillCenter for Phage Technology, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0002-9494-6053
Arum HanDepartment of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0002-9223-8301

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The exploration of bacteriophage diversity remains constrained by reliance on conventional plaque assays, which bias discovery toward phages that form visible plaques. We present PRISM (Phage Recovery and Investigation in Single-droplet Microenvironments), a droplet microfluidics-based, plating-independent platform for high-throughput isolation, quantification, and characterization of bacteriophages. By encapsulating phage-host interactions in water-in-oil microdroplets at single-phage resolution and coupling them with fluorescence-based detection and sorting, we demonstrate PRISM's capabilities across several key applications: (i) recovery of both "plaquing" and "nonplaquing"

Indexed as

BacteriophagesMicrofluidicsViral Plaque Assay

Identifiers

PMID41880513
PMCPMC13015883

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.