Evidence map›Paper›PMID 41880330›Full record

ArticleSTAR protocols2026

Protocol for whole-brain immunolabeling, clearing, and light-sheet imaging of C-FOS in the pigeon.

Spencer D Balay, Gregory C Nordmann, Simon Nimpf, E Pascal Malkemper, Lukas Landler, David A Keays

Abstract read
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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Spencer D BalayDepartment of Biology, Ludwig-Maximilians-University Munich, Planegg-Martinsried, 82152 Planegg, Germany. Electronic address: balay@bio.lmu.de.
Gregory C NordmannMax Planck Institute for Biological Intelligence, Planegg-Martinsried, 82152 Planegg, Germany. Electronic address: gregory.nordmann@bi.mpg.de.
Simon NimpfDepartment of Biology, Ludwig-Maximilians-University Munich, Planegg-Martinsried, 82152 Planegg, Germany.
E Pascal MalkemperMax Planck Institute for Neurobiology of Behavior - Caesar, 53175 Bonn, Germany.
Lukas LandlerBOKU University, Institute of Zoology, Department of Ecosystem Management, Climate and Biodiversity, 1180 Vienna, Austria.
David A KeaysDepartment of Biology, Ludwig-Maximilians-University Munich, Planegg-Martinsried, 82152 Planegg, Germany; University of Cambridge, Department of Physiology, Development & Neuroscience, CB2 3EG Cambridge, UK; Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 1030 Vienna, Austria. Electronic address: keays@biologie.uni-muenchen.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advancements in tissue clearing and light sheet microscopy have provided new ways to study neural circuits dedicated to sensory processing in a diverse range of animal species. Here, we present a modified iDISCO+ protocol for whole-brain immunolabeling with the neural activity marker C-FOS in the pigeon brain. We describe steps for whole-brain pre-processing, bleaching, and immunostaining. We then detail procedures for obtaining ultramicroscopic light-sheet images in cleared pigeon brains. For complete details on the use and execution of this protocol, please refer to Nordmann et al.

Indexed as

BrainColumbidaeImmunohistochemistryProto-Oncogene Proteins c-fosAnimalsProto-Oncogene Proteins c-fosModel OrganismsNeuroscienceSystems biology

Identifiers

PMID41880330
PMCPMC13045675

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.