ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2025
Determination of the pyrrolo[2,3-d] pyrimidine antifolate AGF94 from a preclinical murine study using a simple UHPLC-MS/MS assay.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
A UHPLC-MS/MS method for the detection and quantification of the 6-substituted pyrrolo[2,3-d] pyrimidine AGF94, a novel antifolate, in mouse plasma and tissue was developed and validated. The developed method relies on a simple protein precipitation with methanol, followed by separation on a C18 column using a gradient solvent system of acetonitrile and water with 0.1 % formic acid in both. Detection and quantification of AGF94 were achieved by multiple reaction monitoring using a Sciex QTRAP 5500 mass spectrometer operated in positive electrospray ionization mode. The transitions for AGF94 and the internal standard were m/z 448 to 137 and 442 to 295, respectively. The calibration curve ranged from 5 to 500 ng/mL in mouse plasma with a linearity of R
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