Evidence map›Paper›PMID 41878501›Full record

ArticleTransplantation direct2026

Varying Perfusate Hemoglobin During Normothermic Ex Situ Heart Perfusion: Elevated Hemoglobin Exacerbates Functional Decline Through Hemolysis.

Mitchell J Wagner, Guilherme Mainardi Aguiar da Silva, Parham Hassanzadeh, Sanaz Hatami, Xiuhua Wang, Jayan Nagendran, Darren H Freed

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transplantation direct, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mitchell J WagnerDepartment of Surgery, University of Alberta, Edmonton, AB, Canada.ORCID https://orcid.org/0000-0002-5883-6340
Guilherme Mainardi Aguiar da SilvaDepartment of Surgery, University of Alberta, Edmonton, AB, Canada.ORCID https://orcid.org/0009-0006-3293-0976
Parham HassanzadehDepartment of Surgery, University of Alberta, Edmonton, AB, Canada.
Sanaz HatamiDepartment of Surgery, University of Alberta, Edmonton, AB, Canada.ORCID https://orcid.org/0000-0002-6792-6620
Xiuhua WangDepartment of Surgery, University of Alberta, Edmonton, AB, Canada.
Jayan NagendranDivision of Cardiac Surgery, Department of Surgery, University of Alberta, Edmonton, AB, Canada.ORCID https://orcid.org/0000-0001-5827-9823
Darren H FreedDivision of Cardiac Surgery, Department of Surgery, University of Alberta, Edmonton, AB, Canada.ORCID https://orcid.org/0000-0002-6239-1444

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Ex-situ heart perfusion (ESHP) enables improved donor heart preservation, yet still suffers functional decline. The perfusate is 1 area that requires optimization, especially with regard to hemoglobin (Hb) content. We sought to compare performance of concentrated, physiologic, and dilute perfusates in minimally damaged hearts. Methods: Hearts of juvenile Yorkshire pigs (45-55 kg) were procured and subject to 11 h of working-mode ESHP in 1 of 4 groups varying in Hb: concentrated blood (CB, ~12 g/dL Hb, n = 4), whole blood (WB, ~8g/dL Hb, n = 6), plasma (~4g/dL Hb, n = 6, diluted with donor plasma), and control (~4g/dL Hb, n = 5, diluted with modified Krebs-Heinseleit). Functional, metabolic, and hemolytic markers were recorded and compared between groups statistically using appropriate statistical tests. Results: CB hearts exhibited worsened decline from baseline in cardiac index, dP/dT max/min, and stroke work ( Conclusions: Hb dilution appears to lower absolute output but preserves baseline function. Supraphysiologic Hb elevation or sublethal blood cell damage exacerbates hemolysis, which contributes to functional decline during ESHP. Lactate evolution did not appear to associate with the degree of functional loss.

Identifiers

PMID41878501
PMCPMC13008187

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.