Evidence map›Paper›PMID 41876441›Full record

ArticleJournal of microbiology (Seoul, Korea)2026

Inhibitory effects of acetyl-11-keto-β-boswellic acid (AKBA) on human cytomegalovirus (HCMV) in vitro.

Bingquan Chu, Zhiwei Ding, Xinna Wu, Yunchuang Chang, Chunxia Wu, Yicheng Fu, Genxiang Mao, Sanying Wang

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiology (Seoul, Korea), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Bingquan ChuSchool of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, P. R. China.
Zhiwei DingSchool of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, P. R. China.
Xinna WuZhejiang Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou 310030, P. R. China.
Yunchuang ChangZhejiang Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou 310030, P. R. China.
Chunxia WuZhejiang Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou 310030, P. R. China.
Yicheng FuZhejiang Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou 310030, P. R. China.
Genxiang MaoZhejiang Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou 310030, P. R. China.
Sanying WangZhejiang Key Laboratory of Geriatrics and Geriatrics Institute of Zhejiang Province, Zhejiang Hospital, Hangzhou 310030, P. R. China.

Funding

National Demonstration Zones for Comprehensive TCM Reform GZY-ZJ-KJ-24055National Demonstration Zones for Comprehensive TCM Reform KJS-ZJ-2025-074TCM Key Laboratory Cultivation Base of Zhejiang Province TCM-202402
6 · The paper itself

Abstract

This study presents the first investigation of acetyl-11-keto-β-boswellic acid (AKBA)'s anti-human cytomegalovirus (HCMV) activity in vitro and elucidates its underlying mechanisms. In HCMV Towne strain-infected WI-38 cells, AKBA (1-12 μM) exhibited negligible cytotoxicity while significantly suppressing virus-induced cytopathic effects (CPE) at 6-10 μM, with dose-dependent reduction of viral proteins (IE1/2 and p52) expression, viral DNA copy number (UL123, UL44, and UL32), and infectious viral progeny titer (TCID50). Time-of-addition experiments demonstrated the primary antiviral activity of AKBA during post-entry phase, along with direct virion inactivation. Transcriptome analysis revealed that AKBA significantly downregulated the expression of the host factor NR4A1 induced by HCMV, a finding further validated by Western blotting. Further gene knockdown experiments confirmed that silencing NR4A1 significantly reduced the expression of viral proteins IE1/2, thereby validating NR4A1 as a key host factor for HCMV infection. These findings indicate that AKBA has a potent and dose-dependent inhibitory effect on HCMV replication in WI-38 cells, and proves that this effect is mediated through two different mechanisms: one is the downregulation of the expression of the key host factor NR4A1, and the other is the direct inactivation of HCMV viral particles.

Indexed as

Antiviral AgentsCytomegalovirusTriterpenesCell LineCytomegalovirus InfectionsFibroblastsHumansImmediate-Early ProteinsNuclear Receptor Subfamily 4, Group A, Member 1Viral ProteinsVirus Replicationacetyl-11-ketoboswellic acidAntiviral AgentsImmediate-Early ProteinsNuclear Receptor Subfamily 4, Group A, Member 1TriterpenesViral Proteinsacetyl-11-keto-β-boswellic acidantiviralhuman cytomegalovirusNR4A1

Identifiers

PMID41876441
PMCPMC13577096

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.