Evidence map›Paper›PMID 41875894›Full record

ArticleCell reports. Medicine2026

Small molecule-directed differentiation of submerged-cultured human nasal airway epithelia for respiratory disease modeling.

Henriette H M Dreyer, Georgia-Nefeli Ithakisiou, Sacha Spelier, Malina K Iwanski, Eugene Katrukha, Jonne Terstappen, Lisa W Rodenburg, Aditi Shekhar, Loes A den Hertog-Oosterhoff, Shannon M A Smits and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Lung organoids: a new frontier in neonatology and paediatric respiratory medicine.European respiratory review : an official journal of the European Respiratory Society · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Henriette H M DreyerLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Georgia-Nefeli IthakisiouLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Sacha SpelierLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Malina K IwanskiCell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
Eugene KatrukhaCell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands.
Jonne TerstappenCenter for Translational Immunology, University Medical Centre Utrecht, Utrecht, the Netherlands; Department of Pediatrics, Wilhelmina Children's Hospital, University Medical Centre Utrecht, Utrecht, the Netherlands.
Lisa W RodenburgLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Aditi ShekharLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Loes A den Hertog-OosterhoffLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Shannon M A SmitsLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Isabelle S van der WindtLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Lotte T AzinkDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands.
Linda H M BijlardLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Koen PassierLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands.
Sam F B van BeuningenLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands; Centre for Living Technologies, Eindhoven-Wageningen-Utrecht Alliance, Utrecht, the Netherlands.
Robert Jan LebbinkDepartment of Medical Microbiology, University Medical Center Utrecht, Utrecht, the Netherlands.
Eric G HaarmanDepartment of Paediatric Pulmonology, Emma Children's Hospital, Amsterdam UMC, Amsterdam, the Netherlands.
Cornelis K van der EntDepartment of Pediatric Pulmonology, Wilhelmina Children's Hospital, University Medical Centre Utrecht, Member of ERN-LUNG, Utrecht, the Netherlands.
Lukas C KapiteinCell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science, Utrecht University, Utrecht, the Netherlands; Centre for Living Technologies, Eindhoven-Wageningen-Utrecht Alliance, Utrecht, the Netherlands.
Louis J BontCenter for Translational Immunology, University Medical Centre Utrecht, Utrecht, the Netherlands; Department of Pediatrics, Wilhelmina Children's Hospital, University Medical Centre Utrecht, Utrecht, the Netherlands.
Jeffrey M BeekmanLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands; Centre for Living Technologies, Eindhoven-Wageningen-Utrecht Alliance, Utrecht, the Netherlands.
Gimano D AmatngalimLab of Cellular Disease Models, Department of Pediatrics, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht, the Netherlands. Electronic address: g.d.amatngalim@umcutrecht.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Submerged cultures of undifferentiated or transformed epithelial cells are widely used in respiratory research due to their ease of use and scalability. However, these systems fail to capture the cellular diversity of the human airway epithelium. Here, we describe a submerged differentiation model using cryopreserved human nasal epithelial cells obtained via minimally invasive brushings. By targeting Notch and BMP signaling with small molecule inhibitors, we differentiate these cells into complex epithelial cultures containing basal, secretory, and ciliated cell types on standard plastic cultureware. This method supports scalable culture of both 2D epithelial monolayers and 3D organoids and is applied to disease modeling in primary ciliary dyskinesia, cystic fibrosis, and respiratory syncytial virus infection. The resulting system enables scalable assessment of disease-relevant epithelial functions in respiratory research.

Indexed as

Cell DifferentiationEpithelial CellsModels, BiologicalNasal MucosaSmall Molecule LibrariesCell Culture TechniquesCells, CulturedCystic FibrosisHumansOrganoidsReceptors, NotchRespiratory Syncytial Virus InfectionsSignal TransductionReceptors, NotchSmall Molecule Librariesairway epithelial cell culturesairway organoidscystic fibrosishigh-throughput epithelial screeningmucociliary epitheliumnasal epithelial cellspatient-derived airway epithelial modelsprimary ciliary dyskinesiarespiratory syncytial virusRSVsubmerged differentiation

Identifiers

PMID41875894
PMCPMC13130688

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.