Evidence map›Paper›PMID 41875815›Full record

SynthesisPoultry science2026

Interactive effects of dietary total sulfur amino acids and crude protein on growth performance in broiler chickens: Meta-analysis and experimental validation.

Hyun Woo Kim, Dong Yong Kil

Abstract readMeta-AnalysisValidation Study
In one paragraph

Synthesis in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Hyun Woo KimDepartment of Animal Science and Technology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of Korea.
Dong Yong KilDepartment of Animal Science and Technology, Chung-Ang University, Anseong-si, Gyeonggi-do 17546, Republic of Korea. Electronic address: dongyong@cau.ac.kr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study conducted the meta-analysis of interactive effects of dietary total sulfur amino acids (TSAA) and crude protein (CP) on average daily feed intake (ADFI), average daily gain (ADG), and feed conversion ratio (FCR) in broiler chickens, aiming to estimate phase-dependent optimal digestible TSAA levels across dietary CP levels. Moreover, the optimal digestible TSAA levels with different CP levels, which were identified in the meta-analysis, were further validated using a broiler feeding trial. In the meta-analysis, a total of 37 studies published between 2007 and 2024 were included. An initial full quadratic model with CP and digestible TSAA showed limited significance of model coefficients with little biological interpretation across growth phases. Therefore, a simplified quadratic model was employed, incorporating the product of digestible TSAA and CP (TSAA × CP) as a single interactive term with quadratic effects. The results indicated significant coefficients (P < 0.05) for ADG and FCR in the starter phase, ADFI and FCR in the grower phase, FCR in the finisher phase, and ADG and FCR in the overall growth phase. Nonlinear interactions for FCR were consistent across all phases. Model performance evaluations also indicated that FCR was the most reliable variable for predicted responses, exhibiting the least prediction errors and between-study heterogeneity. Model simulations across fixed CP levels from 16.0% to 24.0% revealed that optimal digestible TSAA levels for ADFI, ADG, and FCR increased as dietary CP decreased, with an increment of approximately 0.02 to 0.07 percentage points in digestible TSAA for each 0.5-percentage point reduction in dietary CP. Experimental validation trial further supported these predictions, demonstrating that diets formulated with the model-derived optimal digestible TSAA levels decreased FCR (P < 0.05) compared with diets following their commercial recommendations, particularly under reducing CP levels. In conclusion, this meta-analysis suggested that optimal digestible TSAA levels required to maximize growth performance in broiler chickens consistently increased with reducing CP levels in diets across all growth phases.

Indexed as

Amino Acids, SulfurChickensDietary ProteinsAnimal FeedAnimal Nutritional Physiological PhenomenaAnimalsDietAmino Acids, SulfurDietary ProteinsBroiler chickenCrude proteinGrowth performanceMeta-analysisTotal sulfur amino acid

Identifiers

PMID41875815
PMCPMC13049512

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.