ArticleBiomedica : revista del Instituto Nacional de Salud2026
Obtention of conditional media from Wharton’s gelatin mesenchymal stromal cells in a dynamic culture system
Article in Biomedica : revista del Instituto Nacional de Salud, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 authors.
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Abstract
Introduction: Mesenchymal stem cells have great therapeutic potential due to their antiinflammatory, anti-apoptotic and pro-angiogenic effects through their secretome. Wharton’s gelatin is one of the best sources of mesenchymal stromal stem cells; their secretome is rich in bioactive molecules with the ability to maintain cellular and tissue homeostasis for the treatment of pathologies such as inflammation, skin wounds, neurodegenerative disorders, and diabetes. Objective: To describe a protocol for obtaining conditioned media from mesenchymal stromal stem cells isolated from Wharton’s jelly through dynamic cell cultures, considering that 3D systems offers advantages over 2D by better replicating the conditions of the cellular microenvironment in vivo. Materials and methods: The mesenchymal stromal stem cells obtained were characterized in vitro: their immunophenotype by flow cytometry and their multipotency by induction of differentiation to mesenchymal lineages. The stem cells were grown in 2D monolayer cultures and 3D cultures, in a 3L Applikon Biotechnology bioreactor using plastic microbeads as microcarriers. Obtention of the conditioned media was standardized and 43 angiogenic factors were detected, using an antibody microarray system for human angiogenesis. Results: Isolated cells showed typical characteristics of mesenchymal stromal stem cells. 3D cultures facilitated scalability and improved secretion of angiogenic factors compared to 2D cultures. Conclusions: The secretome was obtained with 2D and 3D cultures. Significantly larger volumes of conditioned medium with a potentially increased angiogenic capacity were obtained with 3D cultures.
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