Evidence map›Paper›PMID 41875136›Full record

ArticleSTAR protocols2026

Protocol for acute oxidative stress induction in third-instar Drosophila melanogaster larvae.

Jinhua Li, Yanzhe Pan, Yansong Zhang, Siyuan Lin, Min Liu, Alan Jian Zhu

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jinhua LiState Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China. Electronic address: jinhua@stu.pku.edu.cn.
Yanzhe PanState Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China.
Yansong ZhangState Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China; Peking University Chengdu Academy for Advanced Interdisciplinary Biotechnologies, Chengdu, Sichuan 610213, China.
Siyuan LinState Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China.
Min LiuState Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: liumin02@pku.edu.cn.
Alan Jian ZhuState Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, China; Peking University Chengdu Academy for Advanced Interdisciplinary Biotechnologies, Chengdu, Sichuan 610213, China; Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. Electronic address: zhua@pku.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol for inducing oxidative stress in the Drosophila melanogaster larval brain to facilitate the screening of regulators involved in redox homeostasis. We describe steps for the collection of synchronized larvae and their treatment with oxidative stressors. We then detail procedures for larval brain dissection, lipid droplet staining, image acquisition, and the assessment of glutathione redox status. For complete details on the use and execution of this protocol, please refer to Li et al.

Indexed as

Drosophila melanogasterOxidative StressAnimalsBrainGlutathioneLarvaOxidation-ReductionGlutathioneCell BiologyDevelopmental biologyGeneticsMetabolismModel OrganismsMolecular Biology

Identifiers

PMID41875136
PMCPMC13045674

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.