Evidence map›Paper›PMID 41871104›Full record

ArticleSTAR protocols2026

Protocol to purify fatty acid metabolites with two approaches for quantification using liquid chromatography-mass spectrometry.

Tomomi Hashidate-Yoshida, Masaki Yamada, Katsuyuki Nagata, Yasuyuki Kihara, Yoshihiro Kita, Hideo Shindou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Tomomi Hashidate-YoshidaDepartment of Lipid Life Science, National Institute of Global Health and Medicine, Japan Institute Health Security (JIHS), Shinjuku-ku, Tokyo 162-8655, Japan.
Masaki YamadaShimadzu Corporation, Nakagyo-ku, Kyoto 604-8511, Japan.
Katsuyuki NagataDepartment of Lipid Life Science, National Institute of Global Health and Medicine, Japan Institute Health Security (JIHS), Shinjuku-ku, Tokyo 162-8655, Japan; Department of Molecular Biology, The Jikei University School of Medicine, Minato-ku, Tokyo 105-8461, Japan.
Yasuyuki KiharaCenter for Neurologic Diseases, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA 92037, USA.
Yoshihiro KitaLife Sciences Core Facility, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
Hideo ShindouDepartment of Lipid Life Science, National Institute of Global Health and Medicine, Japan Institute Health Security (JIHS), Shinjuku-ku, Tokyo 162-8655, Japan; Department of Medical Lipid Science, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan. Electronic address: shindou.h@jihs.go.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fatty acid metabolites, such as eicosanoids and docosanoids, play important biological roles and are strictly regulated by diverse enzymes. Here, we present two approaches for purifying their metabolites, including the phospholipid mediator platelet-activating factor (PAF), for quantification using liquid chromatography-mass spectrometry (LC-MS). We describe steps for tissue collection, lipid extraction, and lipid mediator purification. We then detail procedures for data analysis. This protocol allows the selection of a suitable technique for analyzing target molecules. For complete details on the use and execution of this protocol, please refer to Yamamoto et al.

Indexed as

Fatty AcidsLiquid Chromatography-Mass SpectrometryMetabolomicsAnimalsHumansPlatelet Activating FactorFatty AcidsPlatelet Activating FactorCell MembraneHealth SciencesMetabolismSignal Transduction

Identifiers

PMID41871104
PMCPMC13049510

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.