ArticleJournal of synchrotron radiation2026
Critical point drying of brain tissue for X-ray phase-contrast imaging.
Article in Journal of synchrotron radiation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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16 authors.
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Abstract
X-ray phase-contrast tomography can efficiently image brain tissue at subcellular resolution. However, current sample preparation methods are not optimized to exploit the full potential of X-ray contrast mechanisms. Here we propose to replace interstitial material by air to enhance X-ray phase contrast of the ultrastructural features. Critical point drying (CPD) of heavy-metal-stained mouse brain tissue produced samples with preserved ultrastructure, a nanofoam-like material that remains compatible with follow-up conventional resin embedding. Using two synchrotron-based setups, namely, a high-throughput microtomography beamline and a nanoscale holographic tomography beamline, we found that CPD samples consistently showed 2-4× stronger phase-shift signal than samples embedded in resin. CPD offers a versatile route for preparing tissue for subcellular and ultrastructural-resolution X-ray imaging. It retains structural detail while improving signal, and is compatible with follow-up protocols involving femtosecond laser milling or electron microscopy, paving the path for biological tissue imaging beyond the mm
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