Evidence map›Paper›PMID 41870679›Full record

ArticleCellular and molecular life sciences : CMLS2026

Heparan sulfate as an attachment factor for ASFV is restricted by host HB-EGF.

Wenchao Xue, Fayu Yang, Zhonghui Zhang, Xiangle Zhang, Yuxi Yang, Yuxuan Li, Zhengshan Feng, Yong Li, Xi Lan, Haixue Zheng and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Wenchao XueState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Fayu YangState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Zhonghui ZhangState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Xiangle ZhangState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Yuxi YangState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Yuxuan LiState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Zhengshan FengState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Yong LiState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Xi LanState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Haixue ZhengState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China.
Zixiang ZhuState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China. zhuzixiang@caas.cn.
Shilei ZhangState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou University, Lanzhou, 730000, China. ZhangSL8600@outlook.com.

Funding

National Key R&D Program of China 2025YFD1800600
6 · The paper itself

Abstract

African swine fever virus (ASFV) is a highly virulent pathogen that poses a significant threat to the global pig industry, and no vaccines or effective treatments are currently available. Cellular attachment is initial and crucial for viral entry and subsequent infection. Understanding the molecular mechanisms of ASFV entry is therefore essential for developing antiviral strategies. Here, we show that cellular heparan sulfate (HS) assists ASFV attachment and subsequent entry into the target cell. Chemical inhibitors of HS and competition assays using heparin and heparan sulfate impair ASFV attachment and infection in the cultured cells. The direct binding between the ASFV virion and heparin is demonstrated by using heparin-coated beads, and this interaction can be blocked by free heparin. In addition, we identify host HB-EGF as a restriction factor against ASFV and reveal that the heparin-binding domain (HBD) is critical for its antiviral activity. The HBD-derived peptide exhibits strong binding affinity for heparin and exerts potent antiviral activity against ASFV. Together, our findings indicate that HS acts as an attachment factor for ASFV, and pharmacologically targeting it may represent a potential strategy for antiviral development.

Indexed as

African Swine FeverAfrican Swine Fever VirusHeparan SulfateHeparin-binding EGF-like Growth FactorVirus AttachmentAnimalsChlorocebus aethiopsHeparinHumansProtein BindingSwineVero CellsVirus InternalizationHeparan SulfateHeparinHeparin-binding EGF-like Growth FactorAfrican swine fever virusHB-EGF.Heparan sulfateViral attachment

Identifiers

PMID41870679
PMCPMC13046906

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.