Evidence map›Paper›PMID 41869569›Full record

ArticleiScience2026

Functional and metabolomic analyses of brown adipose tissue during cold-deacclimation reveal rapid N-acetylated amino acid adaptations.

Chantal A Pileggi, Ella McIlroy, Lauren M K Hamilton, Nidhi Kuksal, Luke S Kennedy, Valeria Vasilyeva, Michel N Kanaan, Ziyad El Hankouri, Yan Burelle, Miroslava Cuperlovic-Culf and 1 more

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Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Chantal A PileggiDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Ella McIlroyDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Lauren M K HamiltonDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Nidhi KuksalDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Luke S KennedyDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Valeria VasilyevaDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Michel N KanaanDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Ziyad El HankouriDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Yan BurelleInterdisciplinary School of Health Sciences, Faculty of Health Science, University of Ottawa, Ottawa, ON, Canada.
Miroslava Cuperlovic-CulfDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.
Mary-Ellen HarperDepartment of Biochemistry, Microbiology, and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Non-shivering thermogenesis (NST) in brown adipose tissue (BAT) is rapidly activated in the cold but inactive at warm ambient temperatures. To elucidate the metabolic remodeling in BAT during recovery from cold exposure, mice were acclimated to 4°C for 7 days, then deacclimated at thermoneutrality (30°C) for 3-48 h. Cold-acclimated mice demonstrated high metabolic rates and food intake, which decreased immediately by ∼40% upon deacclimation. Uncoupled respiration decreased by 24 h, corresponding with gradual declines in mitochondrial protein content and UCP1 gene expression. Decreases in BAT mitochondrial content paralleled declines in protein content by 48 h of cold deacclimation. Metabolomic profiling revealed major alterations in amino acid, TCA cycle, glutathione, and purine metabolism pathways. Marked decreases in the abundance of N-acetylated amino acids in cold deacclimated mice corresponded with increased aminoacylase 1 (Acy1) expression. Together, results highlight the coordinated structural and metabolic remodeling of BAT mitochondria during thermogenesis and deactivation.

Indexed as

physiology

Identifiers

PMID41869569
PMCPMC12999349

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.