ArticlePeerJ2026
Integrative metabolomics using untargeted UHPLC-MS/MS and chemometrics identifies optimal maturity stage of
Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Selective elimination of amyloid-β-induced senescent neuroblastoma cells by Moringa oleifera leaf extract.Scientific reports · 2026Article
- Modern genomic and omics-based technologies for millet breeding and genetic improvement.Frontiers in plant science · 2026Review
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Methods: Results: Day-60 leaf extracts exhibited higher TPC and TFC compared to younger stages, with strong antioxidant activity as indicated by elevated DPPH and FRAP values. In contrast, AChE inhibition was highest in day-30 extracts. Metabolomics profiling with chemometrics integration using MVDA identified and tentatively annotated 27 metabolites with predominantly flavonoids (59%), phenolic acids (19%), glucosinolates (7%), and minor components including coumarins, folates, and alkaloid-like compounds. Principal component analysis (PCA) and partial least squares (PLS) biplot revealed distinct metabolic clustering, with day-60 extract formed clear separated cluster, determined by accumulation of quercetin rutinoside, chlorogenic acid, kaempferol derivatives, and procyanidin B2. PLS biplot demonstrated that quercetin derivatives, chlorogenic acid, and procyanidin B2 were positively associated with antioxidant indicator, while coumarin and folic acid aligned with AChE inhibition. Unknown metabolites indicated by chromatogram peak area with high Variable Importance in Projection (VIP) scores (>1) also contributed to bioactivity trends and separation, lead to potential of unannotated phytochemicals. Conclusion: This study found that maturity stage influences the phytochemistry and bioactivities of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.