Transcriptomic profiling of wheat (

Introduction: The wheat blast fungus Methods: We performed dual RNA-seq analysis of MoT-inoculated wheat leaves at 0, 24, 36, and 48 hpi, mapping reads separately to the wheat and Results: Wheat exhibited pronounced stage-specific transcriptional reprogramming, with peak differential gene expression at 36 hpi and visible symptoms at 48 hpi. The 24 hpi stage was characterized by rapid induction of immune- and defense-related pathways, including innate immunity and detoxification processes, along with downregulation of cell wall and membrane biosynthesis. By 36 hpi, wheat maintained sustained activation of immune and detoxification pathways, while chloroplast- and photosynthesis-associated genes were broadly repressed, consistent with transcriptional features of metabolic constraint. At 48 hpi, coinciding with lesion initiation, transcriptomes showed persistent, metabolically costly immune and defense responses together with extensive suppression of photosynthesis- and chloroplast-associated functions, which were associated with metabolic strain and a transition toward necrosis. Analysis of pathogen-derived reads revealed temporal induction of multiple effector candidates, including known Discussion: Together, these findings provide a temporal framework for wheat blast susceptibility and highlight key host pathways and effector candidates that define critical windows for functional dissection of MoT virulence and wheat susceptibility.