Evidence map›Paper›PMID 41868395›Full record

ArticleFrontiers in veterinary science2026

Development of a colloidal gold immunochromatographic strip for the rapid detection of fowl adenovirus serotype 4 using prepared penton protein-specific monoclonal antibodies.

Sisi Luo, Bingyi Yang, Jiaoling Huang, You Wei, Zhixun Xie, Xiaofeng Li, Aiqiong Wu, Zhihua Ruan, Sheng Wang, Yanfang Zhang and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Sisi LuoInstitute for Poultry Science and Health, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, China.
Bingyi YangKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Jiaoling HuangKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
You WeiKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Zhixun XieKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Xiaofeng LiKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Aiqiong WuKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Zhihua RuanKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Sheng WangKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Yanfang ZhangKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Meng LiKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Liji XieKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Ming YanKey Laboratory of China (Guangxi)-ASEAN Cross-Border Animal Disease Prevention and Control, Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Weiwei WangInstitute for Poultry Science and Health, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, China.
Ping WeiInstitute for Poultry Science and Health, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fowl adenovirus serotype 4 (FAdV-4) is the primary pathogen responsible for hydropericardium-hepatitis syndrome (HHS) and is associated with high mortality rates (20-80%) in 3-6-week-old chickens. This study aimed to develop a rapid and specific method for detecting FAdV-4. Two monoclonal antibodies (mAbs 6B3 and 8G11) against the FAdV-4 penton protein were successfully generated using hybridoma technology, both of which exhibited high titers (1:100,000) and strong serotype specificity. Specificity analysis confirmed that these mAbs recognized 12 FAdV-4 isolates from diverse origins without cross-reactivity to other FAdV serotypes or common avian pathogens. On this basis, a colloidal gold immunochromatographic assay was developed; systematic optimization of its key parameters yielded an optimal labeling pH of 8.3, an antibody labeling concentration of 7.2 μg/mL, and optimal coating concentrations for the test line (T-line) and control line (C-line) of 2.5 mg/mL and 1.5 mg/mL, respectively. Performance evaluation of the method demonstrated that it achieved a detection sensitivity of 7.81 × 10

Indexed as

colloidal gold immunochromatographyfowl adenovirus serotype 4monoclonal antibodypenton proteinrapid diagnosis

Identifiers

PMID41868395
PMCPMC12999416

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.