ArticleFrontiers in microbiology2026
A hidden reservoir of antibiotic resistance genes: transferable plasmids in community air and wastewater.
Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Plasmid-mediated dissemination of antibiotic resistance genes (ARGs) poses a major public health threat. In contrast to the well-studied resistance plasmids within pathogens, those from non-pathogenic environmental reservoirs remain underexplored. Here, we characterized transferable multidrug-resistant plasmids captured from community air and wastewater via conjugation assays. Transconjugants obtained from these environmental samples were profiled phenotypically against 17 antibiotics and genetically via short- and long-read sequencing. Conjugative plasmid transfer was successfully captured from 33 (20.6%) of 160 environmental samples, yielding 78 transconjugant isolates and 40 plasmid types. The captured plasmids conferred resistance to 4-18 antibiotics, with near-universal resistance to ampicillin (98.7%) and retained susceptibility to polymyxin B (84.6%). Among 150 ARG instances identified across 19 classes,
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